BYU iGEM/Notebook/11 February 2011
We've designed our forward and reverse primers to clone out the ara-C gene and pBAD promoter for our thermoswitch plasmid.
ara-C pBAD Forward Primer:
ara-C pBAD Reverse Primer: cggCTGCAGaaaaacgggTatggagaaaca
Redesigned the multiple cloning site (MCS) map in light of some new information regarding the OxyR binding site. Click image below to see details.