Ben Shen:E. coli genetics

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oriT – origin of conjugal transfer

ColE1 – high-copy version of ColE1 replicon, for replication in E. coli and other enterobacteria

sacB - Counter-selectable marker, conferrs sucrose sensitivity

Plac-lacZ a - Consitutive expression in lacI negative strains, lacZ a has a multicloning site, useful for blue white screening, or replace lacZ a with your gene using yeast recombination

p15a - Lower copy replicon for E. coli and other enterobacteria

P BAD - L-arabinose inducible promoter system, includes the araC regulator

lacI Q - Negative regulator of Ptac in the absence of inducer.

RK2 - The minimal RK2 replicon. Incompatability group P. Replicates at low copy numbers in over thirty species of Gram-negative bacteria.

pSC101 - Low copy replicon for enterobacteria. Compatible with ColE1, p15a, RK2, pBBR1 and other replicons. This version is unable to efficiently replicate at 37°C or above and can thus be used as a conditional suicide-vector.

pWV01 - Low copy replicon for Gram-positive and Gram-negative bacteria. Known to replicate in E. coli , Staphylococcus sp , Streptococcus sp , Lactococcus sp . and probably others. This verion is temperature sensitive, such that cells with this plasmid should be grown at room-temperature to 30°C. This replicon has been used for allelic replacement, transposon delivery systems, and as a shuttle vector.

pBBR1 - Medium copy replicon for Gram-negative bacteria. Has a very broad host range and is compatible with a wide variety of plasmids.

rpsL - E. coli rpsL gene, codes for ribosomal protein S12. rpsL confers sensitivity to streptomycin, and can be used as a counterselectable marker.

aacC1 - Gentamycin resistance determinant.

T1T2 - Terminators from rrnB from E. coli.

bla - Ampicillin resistance determinant

aphA - 3 - Kanamycin resistance determinant, useful in Gram-positive and Gram-negative bacteria

kanMX - Kanamycin resistance determinant, useful in Gram-negative bacteria, does not confer kanamycin resistance to Staphylococcus aureus , does confer G418-sulfonate resistance to Saccharomyces cerevisiae .

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