Berglund:Kinasing

From OpenWetWare

Jump to: navigation, search

Kinasing the 5' end of purchased RNA oligomers

  • 1µl 10X kinase buffer
  • 5µl water
  • 1µl Rnase inhibitor
  • 1µl 2µM RNA (fc=.2µM or 2pmoles total)
  • 1µl T4 kinase enzyme
  • 1µl gamma-ATP (Perkin-Elmer(NEN) BLU-502z)

Incubate at 37°C for 1 hr.

Clean with BioRad microspin columns.

Run RNA on denaturing gel.

update 9/24/08- Leslie

I never bother to use a spin column before I gel purify. Works great for me.
Personal tools