Berglund:Protein Buffers

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2X SDS loading dye

  • 1 mL 1M Tris-HCl pH 6.8 (fc=50mM)
  • 42 mL 10% SDS (fc=2%)
  • 1 mg Bromophenol Blue (fc=0.1%)
  • 2 mL 100% glycerol (fc=10%)
  • 1 mL 2M DTT (fc=100mM)


5x SDS loading dye (by Julien)

  • 12.5 mL of 1M Tris pH 6.8 (f.c.=50mM)
  • 100 mg of Bromophenol Blue (f.c.=0.2%)
  • 25 mL of 100% Glycerol (f.c.=10%)
  • 3.85 g of DTT (f.c.=100mM)
  • 5 g of SDS (f.c.=2%)
  • H2O up to ~50 mL

Note: f.c. values are the final 1X concentrations (NOT the 5X concentrations).

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