Berglund:Worm Fix

From OpenWetWare

Jump to: navigation, search

Fixing and preserving your worms

Materials

70% ethanol

10% phosphate buffered formalin
4g NaH2PO4*H20
6g Na2HPO4
100ml 40% formaldehyde
900ml sea water or distilled water

Methods

Soon after collecting the worm (the fresher the better, alive is good too)

  1. wash it off
  2. Take tube of 10% phosphate buffered formalin
  3. Place worm into tube (straight up and down if you can)
  4. allow to sit at room temperature for 2 days (48hrs)
  5. remove worm from tube
  6. place worm into new tube that is empty
  7. add 20ml (volume with worm) of 70%ethanol
  8. gently revolve the worm in the ethanol until it is rinsed off
  9. dispose of ethanol, repeat two more times
  10. place worm into new tube, add 70% ethanol to top.
  11. that's it.
Personal tools