Biology 210 at AU:Notebook/Notebook 1/Entry Base

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  • Day 1: Visit Transect to Study and Obtain Sample for Hay Infusion

Site of Transect: In the front of a Church on Massachusetts Ave. Observations: General: In the 20x20 section that group 5 was given was in a very dark shady area. The darkness was from the church and the trees in the area. Trees: There are three large trees in the area. The first one was a holly tree with scant amount of red berries on it and was healthy looking/damp from the previous days rain storm. The second tree was the tallest which appeared to be dead. There was areas where the bark was missing, there was no leaves or evidence of recent leaves on this tree. The last tree is a pine tree with lots of pine needles still attached and healthy looking. This tree was also damp from the rain. Ground: On the ground there was lots of ivy that covered nearly the whole 20x20 area that was provided. There was lots of leaves that were from the trees, including the spiky brownish lifeless pine needles, maple leaves, acorns, and holly leaves. There was lots of small branches of each of the trees noted in this area as well. Under the ivy there was soil, mulch and vines from the trees and the ivy. There were no animals/insects noted in the area that were observed during this initial visit to the intersect.

Abiotic: water, soil, air, minerals in the dirt

Biotic: trees, fungi on the tree trunks, leaves, ivy

Sample Collected/Hay Infusion Culture: We collected the sample with the approval of TA Ben. In the sample we were sure to include about 50% of the dirt/soil and the other the leaves/needles/vines and ivy. We collected this sample in a 50ml conical tube using metal shovels. Then 11.99g of the sample was measured out and placed into a labeled pastic jar which had 500ml of water in it. After adding 0.1g of dried milk to the sample in the water, it was gently mixed by swirling for approximately 10 seconds.



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