Biomod/2011/Harvard/HarvarDNAnos:LabNotebook Nick/2011/06/17

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Friday (2011-06-17)

  • This morning I spent a lot of time working on the Project section of our wiki. Check it out at HarvarDNAnos Project Page!
  • I also investigated the activity of the Restriction Enzyme NOT1 and began designing a mechanism of incorporating its recognition sequence into the seam of the sphere in order to facilitate opening of the sphere. In specific, I designed a construct to test whether or not a restriction enzyme motif would be sufficient to open our sphere. Note in the image below, if the hairpin is open we should be able to detect this on a gel, and this will show that a given amount of pre-cut excess is sufficient to allow a R.E. to cut but sphere to open.

Testing NOT1 Restriction Enzyme

Our Week 2 Team Photo!