Biomod/2011/TUM/TNT/LabbookA/Preparing samples for transmission electron microscope

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Preparing Samples For Transmission Electron Microscope

  • glow-discharge grids at 30mA for 45s
  • add 1µl 5M NaOH to 200µl 2% uranyl acetate staining solution
  • hide solution from light
  • vortex 1minute
  • centrifuge for 2.5 minutes at maximum speed
  • apply 5µl sample on grid
  • incubate for 3 minutes
  • remove liquid from grid without touching its sourface
  • lay grid on 20µl staining solution, then remove stain immediatly from grid
  • lay grid on another drop of 20µl staining solution
  • incubate for 40s seconds
  • remove liquid from grid
  • let grid dry for at least 15 minutes