Biomod/2012/Columbia/KinesinKings/Results

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Kinesin Kings Test

Results

We found that the rhodamine labelled microtubules formed three dimensional micro meshes, with many microtubules “standing up” or orienting themselves perpendicularly to the plane of the flow cell surface. The meshes spanned the height of the flow cell, and to our knowledge, this is the first time that three dimensional structures have been created through the poly-L-lysine- microtubule interaction.

Details

Mechanistically, in order to fully explain the true interactions between the polylysine monolayer and the rhodamine microtubules, a more isolated study needs to be completed. However, in order to observe a potential mechanism, time lapse imaging was done immediately after the flowing of the microtubules. The microtubules initially started out in solution, where the plane of imaging showed certain microtubules becoming anchored to something outside of the plane of focus. However, as the time increased, different microtubules began attaching to the anchored microtubule, and coming into the plane of view. Thus, during the time lapse, we were able to observe single microtubules flowing into view and becoming anchored through non-fluorescing poly-L-lysine in solution. After the 10 minute time lapse was over, it was the same aggregation of microtubules was observed throughout the flow cell, creating the three dimensional macroscopic structure mentioned above. The limiting factor with this method of imaging was that only a single plane within the flow cell could be focused at once during the imaging, which can only give a microscopic explanation of the movements of specific microtubules.


Kinesin Kings Test

Columbia

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