(1) Solutions Adding (to wells)
(2) Subculturing + Seeding cell
cell count: 315
total cell no.=6.3*10^6
4 well plate: 1x10^4 cells per well (*Remarks:it is 5x105 in protocol)
volume of cell solution added:16ul/well
After adding cells to solutions according to the table above, the wells are stored in incubator for 24hrs.