Biomod/2012/HKBU/BU Magician:Labook:Notebook/Labbook/2012/09/13

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2012/09/13

Cell Treatment

(1) Solutions Adding (to wells)
Image:HKBU2012Table5.jpg

(2) Subculturing + Seeding cell
cell count: 315
total cell no.=6.3*10^6
4 well plate: 1x10^4 cells per well (*Remarks:it is 5x105 in protocol)
volume of cell solution added:16ul/well


(3)SiRNA delivery
After adding cells to solutions according to the table above, the wells are stored in incubator for 24hrs.

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