Biomod/2012/Titech/Nano-Jugglers/Methods/EDAC conjugation

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EDAC conjugation

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Overview

    EDAC conjugation is the method to conjugate animo modified DNA onto carboxylated polystyrene surface.Using this method, we can connect platinum to aircraft body part of polystyrene regiospecifically.

Method

    EDAC is known as water-soluble carbodiimide (WSC) and is used as coupling agent to form amide bond. EDAC crosslinks nucleic acids to the surfaces, which have carboxy group. In this project, we used this method to conjugate amino modified DNA onto carboxylated polystyrene-beads. EDAC reacts with carboxylated polystyrene-beads to form active intermediate on the body. Then it reacts to amino modified DNA. The beads are coupled by nucleic acid. We divide the body into three parts including polystyrene surface, so it is possible to connect platinum regiospecifically.
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