Biomod/2014/fit test.html

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Abstract

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Material
Structural formula Name Manufacturer Grade
Sulfo-NHS DOUJINDO 200mg
Tetraethyl orthosilicate
(Tetraethoxysilane; TEOS)
Wako 500mg
3-aminopropyltriethoxysilane Wako 25g
1 - (3-dimethylaminopropyl) carbodiimide hydrochloride TGI 5g
N-N-dimethylformamide TGI 200mg
Succinic anhydride Wako 25g
Cetyltrimethylammonium bromide Wako 25g
MES DOUJINDO 25g



Table 3
Fluorescent moleculePlaceExcitation wavelengthMaximum fluorescence wavelength
TAMRA(red)Sword550nm580nm
FITC(green)Barrel498nm522nm



                 
Apparatus
Device name Manufacturer Model number Measurement condition Photograph Explanations of equipment
Spectrophotometer HITACHI F-2500 WL Range: 450nm~650nm Scanning Speed: 60nm/min This device can see the concentration of the solution, the material characteristics, the structure of the molecule.
SEM KEYENC VE-7800 WL Range: 450nm~650nm Scanning Speed: 60nm/min It is possible to significantly change the magnification easily. In addition, photography is possible.
Confocal laser scanning microscope (CLSM) Nikon ECLIPSE-Ti Ar-Laser (488nm) Garvano mirror scanner It is possible to obtain a high resolution image in focus in the field of view for the entire sample surface with irregularities. Three-dimensional shape measurement non-destructive, non-contact is possible




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