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--David Roche 10:55, 13 August 2009 (EDT)
PCR monkey

  • Colanic acid assay 2.1 protocol - completed
  • Completed miniprep and confirmed that BioBricks taken out of registry were correct.

  • Charles Fracchia 13:41, 13 August 2009 (EDT):
  • Explained thoroughly assays and genetic circuits
  • Started modelling on LacI repressible promoter
  • Designed site directed mutagenesis primers for PAH serine 16 -> Glutamine
  • Sacrificed a sheep for good luck
  • Beefed up the description of PAH activity assay and added step 0 of sonication to the protocol
  • Helped loading Gel electrophoresis for biobrick checking

  • Kun Xue 15:25, 13 August 2009 (EDT):
  • Researched on cI protein sequences and how the Harvard cI sequence might be wrong
  • Beefed up description of Cellulase assay and output data

Royah Vaezi:

  • Midi-prep for PAH. Last steps to be completed tomorrow.
  • PCR of OtsA and Dam. Re-did OtsB PCR using mid-temp PCR product from James C's last round of PCR. Will run all PCR products gained on a gel tomorrow AM to check.
  • Restriction digest of B3023 with XbaI and SpeI (overnight). Vector will be digested tomorrow ready for ligation. Aim is to have the BioBrick on Tues...
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