Chang Lab:Notebook/Assessment of Penetration of Biodegradable Nanopolymers into Biofilm/Entry Base

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Protocol

Date: 3 December 2008; Time: 1100; Agenda: Preparation of Ecoli Inoculum

Date: 4 December 2008; Time: 0930; Agenda: Quadrant Streaking of Petri Dishes prepared on 3rd December

Date: 5 December 2008; Time: 0945; Agenda: Preparation of Ecoli Inoculum and Quadrant Streaking of Petri Dishes

Date: 6 December 2008; Time: 0945; Agenda: Store Petri Dishes that were streaked on 5 December in a 4 degrees fridge.

Date: 9 December 2008; Time: 0900; Agenda: Mid-Log Phase Determination

Date: 13 December 2008; Time: 0915; Agenda: Biofilm formation using M9 and LB Broth.

Date: 15 December 2008; Time: 1400; Agenda: Reprepare M9 medium and quantification of biofilm formed

Date: 16 December 2008; Time: 0830; Agenda: Biofilm formation at 3 temperatures for 48hr.

Date: 17 December 2008; Time: 0830; Agenda: Results of serial plating done on 16 December and sonication done on 15 December.

Date: 18 December 2008; Time: 0930; Agenda: Biofilm formation at 3 temperatures for 24hr.

Date: 19 December 2008; Time: 0930; Agenda: Quantification of 24hr biofilm at 3 temperatures and results of serial plating done on 18 December.

Date: 20 December 2008; Time: 0930; Agenda: Biofilm formation at 3 temperatures for 72hr.

Date: 22 December 2008; Time: 0930; Agenda: Culturing of bacteria to grow biofilm the next day.

Date: 23 December 2008; Time: 0930; Agenda: Quantification of 72hr biofilm at 3 temperatures and biofilm formation at 21deg and 37deg for 72hr.

Date: 26 December 2008; Time: 1400; Agenda: Quantification of 72hr biofilm at 2 temperatures.

Date: 27 December 2008; Time: 0930; Agenda: Biofilm formation at 1 temperature for 48hr and 72hr.

Date: 29 December 2008; Time: 1400; Agenda: Quantification of 48hr biofilm at 1 temperature.

Date: 30 December 2008; Time: 1400; Agenda: Quantification of 72hr biofilm at 1 temperature and biofilm formation at 24hr and 72hr.

Date: 31 December 2008; Time: 1400; Agenda: Quantification of 24hr biofilm at 1 temperature.

Date: 2 January 2009; Time: 1400; Agenda: Quantification of 72hr biofilm at 1 temperature.

Date: 4 January 2009; Time: 1400; Agenda: Streaking of agar plates from slant culture

Date: 6 January 2009; Time: 1400; Agenda: Biofilm formation at 1 temperature for 24hr.

Date: 7 January 2009; Time: 1400; Agenda: Quantification of 24hr biofilm at 1 temperature and biofilm formation at 1 temperature for 24hr and 48hr.

Date: 8 January 2009; Time: 1430; Agenda: Quantification of 24hr and 48hr biofilm at 1 temperature each.

Date: 19 January 2009; Time: 1100; Agenda: Culturing of PA01 inoculum.

Date: 20 January 2009; Time: 1100; Agenda: Culturing of PA01 inoculum.

Date: 21 January 2009; Time: 1100; Agenda: Culturing of PA01 inoculum.

Date: 30 January 2009; Time: 1000; Agenda: Mid Log Phase Determination

Date: 31 January 2009; Time: 1100; Agenda: Mid Log Phase Determination + Results

Date: 2 February 2009; Time:1230; Agenda: Culturing of PA01 inoculum.

Date: 3 February 2009; Time:0800; Agenda: Biofilm formation at 21deg and 30deg for 48hr and 72hr.

Date: 4 February 2009; Time:1200; Agenda: Culturing of PA01 inoculum.

Date: 5 February 2009; Time:0800; Agenda: Biofilm formation at 21deg and 30deg for 24hr + Quantification of 48hr biofilm at 21deg and 30deg.

Date: 6 February 2009; Time:0800; Agenda: Culturing of PA01 inoculum (Redo yesterday's 24hr) + Quantification of 72hr biofilm at 21deg and 30deg.

Date: 23 February 2009; Time:1400; Agenda: Culturing of Ecoli inoculum for SEM.

Date: 24 February 2009; Time:1400; Agenda: Biofilm formation at 37deg, 24hrs for SEM.

Date: 25 February 2009; Time:1400; Agenda: Exposure of biofilm to nanopolymers overnight for SEM.

Date: 26 February 2009; Time:1400; Agenda: Fixing biofilm and airdrying for SEM.


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