This time only using 1 loop instead of the the 5loops for 150ml broth. Reason is to reduce the number of colonies on the agar petri dish so that counting would be more manageable.
Absorbance reading started at 530pm.
Number of time points reduced to 0th hr, 3-5th hr, 14th, 16th and 24hr.
Serial dilution was increased 10^-6, 10^-8 and 10^-10 for (1st 8-hr incubation; while plate 10^-8, 10^-10, and 10^-12 for subsequent hours.
Note
Growth curve of 2nd mid log phase is similar to that of the 1st. Mid log phase of E coli is 5-6th hr.