Chang Lab:Notebook/CBE/08/148/2008/12/10

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Log Phase Trail 2:

1. Repeat the procedure on 9/12.

  • Changes
    • 1 loop of single colony is obtained instead of 5 loops (to reduce the amt of bacteria)
    • Number of time point taken at : 0, 3rd, 4th, 5th, 14th, 16th and 24th hour.
    • Serial dilution from 10^-6 to 10^10 for the first 3 points and 10^18 to 10^-12 for the last 4 points

Note:

  • Growth curve otained as shown below.
Absorbance vs Time (trial II)
Absorbance vs Time (trial II)
    • Mid-log phase ard 5th to 6 th hour...



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