Cong T. Trinh:Double Digestion

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Double digestion

Reaction components

Chemical   	        Volume (μL)
SalI (20,000 U/mL)	5  (Working concentration 100U)
NdeI  (20,000 U/mL)	5  (Working concentration 100U)
10xNEB buffer3	        5
BSA (100x)	        0.5
Template	        (less than 5 ug)
H2O	                ???
Total	                50

Note:

The reaction is carried out at 37oC for 16 hrs.
Avoid to use rare restriction enzymes.



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