Cong T. Trinh:Double Digestion
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Double digestion
Reaction components
Chemical Volume (μL) SalI (20,000 U/mL) 5 (Working concentration 100U) NdeI (20,000 U/mL) 5 (Working concentration 100U) 10xNEB buffer3 5 BSA (100x) 0.5 Template (less than 5 ug) H2O ??? Total 50
Note:
- The reaction is carried out at 37oC for 16 hrs.
- Avoid to use rare restriction enzymes.
