Crook:Media

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Contents

BSE medium (1 L)

  • 1 L dH2O
  • 5 g succinate
  • 500 mg yeast extract
  • 400 mg K2HPO4
  • 400 mg KH2PO4
  • 200 mg MgSO4·7H2O
  • pH to 6.5 with HCl
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave
SOURCE: [1]

DYGS medium (1 L)

  • 1 L dH2O
  • 2 g glucose
  • 2 g yeast extract
  • 1.5 g peptone
  • 1.3 g glutamic acid
  • 500 mg K2HPO4
  • 500 mg MgSO4.7H2O
  • pH to 6.0 with HCl
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave
SOURCE: [2]

LB-Miller medium (1 L)

  • 1 L dH2O
  • 10 g tryptone
  • 5 g yeast extract
  • 10 g NaCl
  • 1 mL 2N NaOH
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

LB-Lennox medium (1 L)

  • 1 L dH2O
  • 10 g tryptone
  • 5 g yeast extract
  • 5 g NaCl
  • 1 mL 2N NaOH
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

LB-Luria medium (1 L)

  • 1 L dH2O
  • 10 g tryptone
  • 5 g yeast extract
  • 500 mg NaCl
  • 1 mL 2N NaOH
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

PH medium (1 L)

  • 1 L dH2O
  • 4 g peptone
  • 0.5 g yeast extract
  • 0.5 g tryptone
  • 0.5 g CaCl2·2H2O
  • 0.2 g MgSO4·7H2O
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

PSY medium (1 L)

  • 1 L dH2O
  • 20 g peptone
  • 20 g sucrose
  • 5 g yeast extract
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave
NOTE: For culturing Bradyrhizobium japonicum, supplement with 0.1% arabinose

PY medium (1 L)

  • 1 L dH2O
  • 5 g peptone
  • 3 g yeast extract
  • 1 g CaCl2·2H2O
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave
SOURCE: [3]

SOB medium (1 L)

  • 20 g tryptone
  • 5 g yeast extract
  • 600 mg NaCl
  • 500 mg KCl
  • 20 mL 1 M Glucose stock
  • 10 mL 2 M Mg2+ stock
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

SOC medium (1 L)

  • 20 g tryptone
  • 5 g yeast extract
  • 600 mg NaCl
  • 500 mg KCl
  • 20 mL 1 M Glucose stock
  • 10 mL 2 M Mg2+ stock
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

TY medium(1 L)

  • 1 L dH2O
  • 6 g tryptone
  • 3 g yeast extract
  • 0.5 g CaCl2·2H2O (this is equal to 3.4 mM)
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

YEM medium (1 L)

  • 1 L dH2O
  • 10 g mannitol
  • 1 g yeast extract
  • 500 mg K2HPO4
  • 200 mg MgSO4·7H2O
  • 100 mg NaCl
    • 3 g agar per 250 mL of broth for agar
    • 1 g agar per 250 mL of broth for top agar
    • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

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