DNA extraction from gel

Gel preparation

Prepare gel with a 1% concentration (i.e. 1.00 gr Agarose low melt point, 100 ml TAE 1X)
Make the wells thicker than usual to fit more DNA into them (you want to maximise the DNA yield, hence minimise the agarose quantity)
Run 1 hour at 90V

Excise the DNA fragment from the agarose gel with a clean, sharp scalpel. Minimize the size of the gel slice by removing extra agarose.
Weigh the eppendorf tubes BEFORE moving the agarose band to them so you can weigh it and assess the gel weight.
Add 3 volumes of Buffer QG to 1 volume of gel (300μL ==> 100 mg)
Incubate at 50° 10 minutes (vortexing every 2-3 minutes helps dissolve agarose)
After the gel slice has dissolved completely, check that the color of the mixture is yellow, it it is NOT add 10 μL Sodium acetate 3M
If your DNA fragment is <500 bp and >4 kb increase the yield by adding 1 gel volume of isopropanol to the sample and mix.
Place a QIAquick spin column in a provided 2 ml collection tube.
To bind DNA, apply the sample to the QIAquick column, and centrifuge for 1 min.

The maximum volume of the column reservoir is 800 µl. For sample volumes of more than 800 µl, simply load and spin again.

Discard flow-through and place QIAquick column back in the same collection tube
(Optional): Add 0.5 ml of Buffer QG to QIAquick column and centrifuge for 1 min. This is to remove all traces of agarose
To wash, add 0.75 ml of Buffer PE to QIAquick column and centrifuge for 1 min. Note: If the DNA will be used for salt sensitive applications, such as blunt-end ligation and direct sequencing, let the column stand 2–5 min after addition of Buffer PE,before centrifuging.
Discard the flow-through and centrifuge the QIAquick column for an additional 1 min at ≥10,000 x g (~13,000 rpm).
Place QIAquick column into a clean 1.5 ml microcentrifuge tube.
To elute DNA, add 50 µl of Buffer EB (10 mM Tris·Cl, pH 8.5) or H2O to the center of the QIAquick membrane and centrifuge the column for 1 min at maximum speed. For increased DNA concentration, add 30 µl elution buffer to the center of the QIAquick membrane, let the column stand for 1 min, and then centrifuge for 1 min.