Dahlquist:SURP 2015 Schedule

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SURP 2015 Schedule

Week Date Wet Lab GRNsight GRNmap Meetings & Events
1 Mon

May 18

Work with GRNmap team Work with GRNmap team AM: How to wiki, PowerPoint overview

PM: Within- and between-array normalization, one-strain ANOVA

  • Anu/Natalie: wt
  • Grace/Monica: Δhap4
  • Kevin M./Nicole: Δgln3
  • Kevin W./KD: Δswi4
  • Tessa/Trixie:Δcin5
  • Note that we chose not to do Δhmo1, Δzap1 or S. paradoxus so that students could work in pairs and check each others' work.
Tues

May 19

Work with GRNmap team Work with GRNmap team AM: Modified T test

PM: Between-strain ANOVA, clustering with stem, preparation of GenMAPP input sheet, "Deception at Duke" video, "Baggerly: Forensic Bioinformatics" video, research ethics discussion

  • SURP BBQ, 12:00-1:00 PM, Bird's Nest
Wed

May 20

Work with GRNmap team
  • Decide which strain to label and hybridize to microarrays: Δino2, Δopi1, or Δyap1
  • Decide which strains to test for growth impairment in cold: Δarg80 (voted no), Δrsf2, Δrtg3, Δtbf1 (essential, null not available), Δyhp1, Δyox1, Δphd1, Δnrg1 or other
Work with GRNmap team GenMAPP, YEASTRACT
2 Tues

May 26

Work with GRNmap team Work with GRNmap team; GRNsight code walk-through All: Create input sheet and run GRNmap

TM/TR: work on issue #74

Wed

May 27

MH/KW: Δyap1 F1 cDNA synthesis, Δyap1 F1 cDNA purification, aRNA synthesis

KM: genotyping PCR Δnrg1

Thurs

May 28

MH/KW: repeat Δyap1 F1 cDNA synthesis, Δyap1 F1 cDNA purification

KM: Δnrg1 PCR gel, Δphd1 genotyping PCR

GJ/NW: work on issue #10
3 Mon

June 1

MH/KW: Δyap1 F1 aRNA synthesis, purification, A260

KM: Δnrg1, Δphd1 temp-sensitive growth expt on plates

Tues

June 2

MH/KW: Δyap1 F1 aRNA gel, dry samples

KM: repeat Δphd1 PCR w/wt template; PCR Δrsf2

Wed

June 3

4 Mon

June 8

KM: Δrsf2, Δrtg3 temp-sensitive growth expt on plates
Tues

June 9

Wed

June 10

  • GRNsight meeting 11:00-12:00 PM, S202
  • Joint group meeting, 12:00-2:00 PM, S202
  • GRNmap meeting 2:00-4:00 PM, S202
5 Mon

June 15

KM: Δyhp1, Δyox1 temp-sensitive growth expt on plates (KD at BioQUEST workshop)
Tues

June 16

(KD at BioQUEST workshop)
Wed

June 17

6 Mon

June 22

Tues

June 23

Wed

June 24

Lab check-out

Lab check-out

Lab check-out

Strain Voting

Δyap1

Δino2

Δopi1


Δrtg3

Δrsf2

Δtbf1

Δyox1

Δyhp1

Δphd1

Δnrg1

Δarg80

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