Dandekar & Chandler:M9 Salts

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10X M9 Salts (per Liter):

  • Na2HPO4 60 g
  • KH2PO4 30 g
  • NaCl 5 g
  • NH4Cl 10 g

Adjust pH to 7.4, autoclave

Before use at 1X add:

  • 2.0 mL of sterile filtered 1M MgSO4
  • 0.1 mL of sterile filtered 1M CaCl2
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