Davidson:Plasmids and Bacteria

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  • Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
  • Create RE biobrick (completed)
  • Isolating genomic DNA from Salmonella typhimurium (completed)
  • Ordered oligos for recombinational enhancer and primers for the hin gene
    • These sequences will be transformed into RFP and possibly GFP plasmids
  • Performed Minipreps to isolate RFP and GFP plasmids to be used for transformations.
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