Davidying: Ideas for M13 Renovation

From OpenWetWare

Jump to: navigation, search

M13 Redesign Ideas

Gene Function Re-engineering ideas
g1 assembly Increase size but maintain shape and interactions, to attempt to create a larger "barrel" to make a wider filament of M13
g2 replication of DNA + strand Separate g2 and g10 by inserting entire sequence of g10 after transcription end of g2/g10 (essentially repeating g10 twice in succession), so that the two genes are independent
g3 phage tail protein Change amino acid sequence to make p3 bind to other proteins besides TolA (perhaps allowing it to infect more bacteria)
g4 assembly (see g1)
g5 binds ssDNA Add GFP or other type of tag to enable rapid measurement of DNA replication (an assay of g2/g10)
g6 phage tail protein Change sequence to allow for easier modification of p3
g7 phage head protein Change sequence to allow for easier modification of p9
g8 phage coat protein Change amino acid sequence to allow g8 (and thus the entire virus) to bind to certain materials, like metals
g9 phage head protein Add or change residues to enable p9 to bind to p3, creating long chains of connected filaments like a nanowire
g10 DNA replication (see g2)
g11 assembly (see g1)
Personal tools