| Gene | Function | Re-engineering ideas
|
| g1 | assembly | Increase size but maintain shape and interactions, to attempt to create a larger "barrel" to make a wider filament of M13
|
| g2 | replication of DNA + strand | Separate g2 and g10 by inserting entire sequence of g10 after transcription end of g2/g10 (essentially repeating g10 twice in succession), so that the two genes are independent
|
| g3 | phage tail protein | Change amino acid sequence to make p3 bind to other proteins besides TolA (perhaps allowing it to infect more bacteria)
|
| g4 | assembly | (see g1)
|
| g5 | binds ssDNA | Add GFP or other type of tag to enable rapid measurement of DNA replication (an assay of g2/g10)
|
| g6 | phage tail protein | Change sequence to allow for easier modification of p3
|
| g7 | phage head protein | Change sequence to allow for easier modification of p9
|
| g8 | phage coat protein | Change amino acid sequence to allow g8 (and thus the entire virus) to bind to certain materials, like metals
|
| g9 | phage head protein | Add or change residues to enable p9 to bind to p3, creating long chains of connected filaments like a nanowire
|
| g10 | DNA replication | (see g2)
|
| g11 | assembly | (see g1)
|
