Project Description/Abstract
Review
Developers
Use Cases, Requirements, Comments
- Top Ten Needed Basic Parts (Parts Comprising the Central Dogma)
- Origins of DNA replication
- Transcriptional Promoters
- Transcriptional Operators
- RNA Processing Elements
- RNA Stability Elements
- Translational Start Sites
- Coding Sequences
- Green Fluorescent Protein
- Translational Pause Sites
- Regulation w/in the trp operon
- Translational Stop Sites
- Protein-Protein Interaction Epitopes
- High-Level Problem Statement
- A BioBrick standard specifies a set of restriction endonucleases that can be used for composing basic parts into composite parts
- A non-standard DNA sequence is likely to have sites in the middle of the sequence that allow for the restriction enzymes specified in the standard to make a cut.
- The algorithm has to identify those sites in the non-standard DNA sequence
- The algorithm has to mutate the sites but must adhere to three constrains
- The mutation eliminates the restriction site (the location where the restriction enzyme makes the cut)
- The mutation does not change the amino acid that is suppose to be encoded if the sequence is an open reading frame
- The mutation does not change the function of the sequence. For example, a mutation in a ribosome binding site (RBS) may lower or increase the affinity of the ribosome to the site
Design
Algorithms
Implementation
Source Code
Tasks
Maintenance
Notes
|
