Endy:Victor3 plate reader/Plates

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The Endy Lab uses plates made by Greiner Bio One.

Path length as a function of volume added to a well

Calculated from the volume of a frustrum using the plate dimensions given in the link above.

Volume (┬Ál) Path Length (cm)
50 0.15
100 0.29
150 0.44
200 0.58
250 7.2
300 0.85
350 0.99
400 1.12

Converting absorbance of a 200μl sample to OD600 using the path length calculated above yields very different numbers than when using a calibration produced from samples measured in both the Endy Lab portable spec and the plate reader.

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