Etchevers:Notebook/Genomics of hNCC/2008/06/25
|Genomics of human neural crest cells|| Main project page|
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Attended lecture by Dominique Gauguier. Using big science tools to get at the genetic bases of common diseases. Combines congenic strain QTL analysis, transcriptomics, proteomics (we didn't hear much about that) and "metabolomics" which is alright, but I preferred the QTL + transcriptomics side of things.
He seems to discount the impact of "trans-" effects on the traits he is studying, be they metabolites or gene transcription level. His website is supposedly http://www.well.ox.ac.uk/fgentcard but the domain seems to be down.
H2 hybridization: Did rinses at 65°C as well as the hybridization, because I had to share the oven. The slides spent 20' in 2x SSC first, then 2x 30' in 50% formamide/2x SSC/0.1% Tween. Followed protocol thereafter. Seminar was after 20' at 37°C in RNAse/ 1x wash solution, so transferred into wash solution in fridge during that time and lunch. Took up with blocking solution 2% + 20% FCS for 1h40, then 1/1000 dilution of anti-dig Fab' for o/n at room temperature.
Signed up for FriendFeed today - for professional reasons. No Flickr photos. Hope to have something to say for the Science Blogging 2008 conference I will be attending in London at the end of August.
Wondering about the effect of FGF2 on the hNCC cultures. Does the dose of FGF2 impact on which receptor it binds (because of different affinities) - and thereby, on which cascade it activates? Eg. am I getting an excess of phospho-ERK that is leading to differentiation rather than just a little bit of JNK activity for example (leading to increased Shh signaling by an intermediate that is currently unclear), or something else that is the target of low these or other MAPKs' activity?