Etchevers:Notebook/Genomics of hNCC/2008/09/03
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Sending myself hNCC
Candice sent yesterday the following vials:
- 1 ampoule de CCN-trunk R1066 passage 20 congelée le 27/12/07 - no cell # noted - 1 ampoule de CCN-cephalic R1066 passage 17 du 08/08/08 - 1.8 x 10^6 cells, this counting before freezing did not thaw well back in May
Procedure for thawing cells
I also found that 2 10cm plate cultures of R1064 (passage 11 from 10-6-08) still had a few (apparently) live stubborn cells in them, even though the last medium change goes back to early July! Morphology much like what I had photographed before, perhaps I will put the photos up somehow. Fairly vacuolar, but don't dead cells all explode? (there was a fair bit of debris). So changed 2/3 of the medium for the fresh medium I just made up, see if I can't kick-start them into dividing again. But quite different cell shape from initial hNCC. Far bigger, to start, lots of actin filaments.