Etchevers:Notebook/Genomics of hNCC/2009/02/27
|Genomics of human neural crest cells|| Main project page|
Previous entry Next entry
First, have just ordered B27 supplement (Invitrogen) because it's in the store, and because I only have enough N1+Stemline Neural +/- retinyl ester supplement as per last week, to last for another couple medium changes or so. And the one with retinyl ester is pretty close to confluent - not looking neuron-like - so may need to be passaged.
Second, have trypsined the Rich or Stemline Neural + serum 25cm2 flasks and the first trypsined much better than the second. Inefficient rinsing? There didn't seem to be an initial density difference, but there were 2x as many cells in the first as in the second:
I am such a noodle, as they say in French. Here I was wondering how I ran out of N-1 supplement so fast. I only diluted it 10x instead of 100x!
Do better with the B-27 supplement, okay? (use up the N-1 first though, as I can not just dilute 10x [| because of the other adjuvants]).
Made fresh Rich medium using a frozen aliquot of the new ES serum.