Etchevers:Notebook/STRA6 in eye development/2008/04/17
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DNA extraction from mouse tails
Angelique is trying to PCR amplify a fragment of mouse genomic DNA that will serve as a template for synthesizing a new RNA probe for in situ hybridization against Pax6.
She kept getting very bad yields. We looked with the spectrophotometer at the genomic DNA concentration and the dilution was way low. We then looked at the stock solution and it was 20x less than indicated. She tried putting all the dilute DNA instead of the water in the next PCR reaction but still a bad yield.
Meantime, I had some frozen pups and was able to break off a bit of tail to extract the DNA.
This evening, all was dissolved except for (presumably) ossifying bits of the tail. What a great principle for a horror novel! Blech.
Drove Kyoko all over town to look for an apartment for her. Only incidentally related to eye project in that she's here to do linkage studies for the high myopia project.