File:012.gif
012.gif (304 × 429 pixels, file size: 16 KB, MIME type: image/gif)
Fig. 1. Scheme of the antigen-mediated genetically modified cell amplification (AMEGA) system. (A) The construct and the activation scheme of the chimeric receptor. The extracellular N-terminal half of EpoR was replaced with ScFv of anti-fluorescein antibody 31IJ3 with a GSG tripeptide linker between ScFv and Val 118 of EpoR D2 domain to create ScFvg. VH and VL regions were linked with a S(G4S)3 flexible linker. Fluorescein dimer addition brings two ScFvg chains into close proximity, leading to signal transduction. (B) Schematic diagram of the selection procedure. Retroviral vectors with long-terminal repeats (LTRs) and a packaging signal () are used for the efficient transcription of ScFvg-IRES-transgene. An immunoglobulin heavy chain secretion signal sequence (S) is located upstream of the chimeric receptor gene to enable their cell surface expression. -from the paper
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 12:37, 12 April 2006 | 304 × 429 (16 KB) | Conversion script (talk | contribs) | (recovered file, missing upload log entry) |
You cannot overwrite this file.
File usage
The following 2 pages use this file: