Freimoser:Recipes:Yeast:YNB vitamins
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1000x vitamin solution for yeast nitrogen base
Remarks:
The recipe described here is based on the description from the John McCusker lab. In contrast to the original recipe I have dissolved pyridoxine and thiamine in water instead of ethanol. Therefore I have added 4 ml EtOH to the ethanol-soluble vitamins in order to keep the volume the same. I have not checked if it is necessary to assure that all vitamins can be dissolved.
Mixing
Volumes for the preparation of 50 ml of a 1000x vitamin stock solution.
| Compound | Stock solution | Vol. for 50 ml 1000x stock |
| biotin | 0.005 g in 50 ml 95% EtOH | 1 ml |
| calcium pantothenate | 0.1 g in 10 ml H2O | 2 ml |
| folic acid | 0.0025 g in 50 ml H2O (warm) | 2 ml |
| myo-inositol | 10% | 1 ml |
| niacin | 1% | 2 ml |
| p-aminobenzoic acid | 0.1 g in 10 ml 95% EtOH | 1 ml |
| pyridoxine HCl | 0.1 g in 10 ml H2O | 2 ml |
| thiamine HCl | 0.1 g in 10 ml H2O | 2 ml |
| riboflavin 5' monophosphate | - | 10 mg |
- remove the individual stock solutions from the refrigerator, may have to warm to get them back into solution
- combine all 95% EtOH vitamin solutions (2 ml), add 4 ml 95% EtOH
- Add 10 mg riboflavin 5' monophosphate (sodium salt dihydrate) to 95% EtOH mixture
- Combine the filtersterilized, water-dissolved vitamin solutions (11 ml) with 33 ml sterile H2O
- Combine the sterile H2O solution with the 95% EtoH solution/suspension for a final volume of 50 ml.
