Restriction Digest and Purification of Plasmid
- Today we ran a restriction digest of a blank vector using Ecor1 and Pst1 restriction enzymes, 10X buffer, and plasmid DNA. After the digest, we purified the DNA using the PCR Purification Kit. This allowed for the DNA to be washed clean of the enzymes. Tomorrow, we will run a gel of the DNA to check our results (mainly to see if we still have DNA and if it was at at the right band length). Also, we will use the digested samples of ATF1, GFP, and the blank vector and ligate them together.
- We then ran an electrophoresis gel to check success and the gel showed that we had no DNA in our sample. This was most likely because we used the Plasmid Miniprep Kit instead of the PCR Purification Kit.