Lambda DNA Aliquots
In order to avoid contamination of the stock solution of Lambda DNA, aliquots must be made.
An aliquot is created by combining the following solutions in a microcentrifuge tube:
Once combined, gently mix the aliquot and store at -20°C.
To use for testing, inject 10 μL of the aliquots into the 500μL Teflon cell volume. This will result in a 1μg/mL DNA concentration for recording translocation events.