Harvard:Biophysics 101/2007/Notebook:HRH/2007-3-13

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Link to assignment:

http://openwetware.org/wiki/Harvard:Biophysics_101/2007/03/13

Task 1

After running the NCBI Blast, I received the following results:

NT_030059.12	Homo sapiens chromosome 10 genomic contig, reference assembly
NW_924884.1	Homo sapiens chromosome 10 genomic contig, alternate assembly (based on Celera assembly)

Additionally, the flanking proteins are:

  • 3895 bp at 5' side: hypothetical protein
  • 425 bp at 3' side: HtrA serine peptidase 1

There is a point mutation at bp 201 (A-T -> A-G).

The reading frame containing the ATG start codon has the mutation changing the amino acid from a CAG to CGG (Glutamine to Arginine). Glutamine is polar and neutral, and Arginine is also polar and strongly basic (they are both hydrophilic).

Laymen's terms: the mutation probably will not do any harm (i.e. don't worry).

Task 2

CAGCCGGGTGACCCAGGCCGAGGCCGGCAGAAGACAGCCTGATGCCTTGAAGACTTCCTCTTGCACTTTT
GTTGGAGGGTGCTGGTTTGCTAAAAGCAGAGAGTATTTTTCTTTTTATTTATTTTGTTTTTAATTTTTTA
ATTTTAGCTCCAGCTCAGTTGCCCAGACTGGAGAGCAGTGGCCAATCATAGCTTACTGCCTCCTGGAACT
CCTGGCTCAATCGATCCTCCTGGATAAGCCTCCTCCGGGTACTATAGGTGTGCACCACCAAGCTCAGCTA
ATTATTTTTTAGAGATGGTTGTCTCTATATGTTGCCCAGGCAGGCCTTGAACGCCTGAGCTCAAGTGATC
CGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCGTCCGGCTATTTTATTTATT
TATAGAGACAGTGTCTCACTTTGTTGCCCAGGCTGGAGTGCAGTGGTACGATCATAGACCACTGCAGCCT
CAAACTCCTGGGCTCAAGTGATCCTCCTGCCTCAGCCTCCTAGAGCAGCTGGGACTACAGATGCACCCCA
CCATGCCTGACTAATTAAAAAAAAATTTTTTTTTAGAGATAGGGTCTTGCTATGTTGCCCTGGCTTAGTT
TTCTTTATGTATGTTTTTTGGGGTTGGAGCTGTAGTGGAACCAGTCCCAGCCCCACTGGGCATCTGGGTC
TGACCTTAGCTGCTCCACTAAGGGCTTAACAGGACACTGGCCACAGGCCTGCTCCCATCCAACTCCACTG
GGGCCACAGGAGGGACCCTACCCAGGATGGAGTGAGGGCAGGCACAGACCAAACAGTGCAGGGTCGGGGG
GTACTGTTCACTGGGACAAGAGATGAGGAAGATGCTGGATTTAAGTGGGCAATGAGGGTGCCTAGCAGAA
GAGGGGACTCTTAACCTGGGTCTTGATGGATGAGTAGAAGTTCTCTGCATGGGAAGGGGTGGAGGTCATT
TCAGGCAGAGGAGGAAGCATTAAACAGCATGGTGTATCTCAAGGAGAGCAGCTGGAGCATGAGTAAGGGG
GGCTGGAGTAGGGGGCAGGGCATGATGTTGGGTCCTGTGGGCCTGGGTTAGCCCCAAGGCAGTCACTGGT
CCTCCACTGACTGTGTGGTCCTCGCTTGGGCTCAGAGGGAAGGGGAGTGATATCCTGGGCTGGTGGGGTG
GATAGCAGCCAACCCACCAAATGGTGGTCACGACCACTGCTGACAACAGTGTGCTGTGTGCTGGGCTGGG
TGTCCACTCTGTCCTCCAGCATCTGAGGCTTGGGATCTCCCAGAAAGTCACACCAGGATGGGAGCCCAGG
TCGTTTGAGCTGGGTGCTCTTGGCTGCTCACTTGCCTTCTTCCTGTCTAGGCCCCTTATAGGTTCACCTG
GCACTGGGACTTGAAGTAGTTATGGCCTGAGGTCTCACTTACCTGGGCTGGCAGTTTTCTGAGTTCGTTC
ATCTCTACCCAGCCCCCGTGGGGCCTTGCGCTCAGTGGCTGTGTGCAGAATGAACCAGTGGAGAGAAAGG
GCTGCACGTAGCGCTCAGGATCCTGGGGTTGGGTTTGTGGCTGAGTGGACCAGGGGCTGACTGAGTGGAG
CCCGTGGCTCTATCTCTCCCTGGGGTCCCACCTGGAAGCCCAAGGCCCTCAGAAAAGGGGTTTCCTTGCC
TGTCATGGCCCTACCCTCCAAGCTGTGTCCTGGATCTTGGCAGTGTCCCCCTGAGCGCCCTGGCTCAGAC
ATCTCCTGCCTGACCGTAGCTGAGCCACGTGCCTTTGTCCTTTATTGCAGCTTCATGGCGGCTGAAGAGA
TGCATTGGCCTGTCCCTATGAAGGCCATTGGTGCCCAGAACCTGCTAACCATGCCTGGGGGCGTGGCCAA
GGCTGGCTACCTGCACAAGAAGGGCGGTACCCAGCTGCAGCTGCTGAAATGTGAGTCCCTGGGGTGGTAG
GGTGCACAGTAGGAGGGCATGGGGGCAGGGCTAGCCATGTAAGTAAGCATCCTCTCCAAGCCTCTGCTTC
CTCACATGGTGTGCCCTCTGGATTTTCCCATTCCCTCTCTGGTGGCTGGCACCCCTGGAGAAGCAGAGGT
TGACTTCCCTAGCCCCCAGGACTGTAGCAGGGTCCGGGGTGGTGCACAGTGCTGGCCAGCTGGCAAGGGG
AGCTGCAGCCTGCAGCCAGGACCTGGGGACCACAGGTGGCCAGGTAGGTCTGGCCTCTGGTGCTGCCCTC
ACTGAATCCGTGAATGTTCAGTGAGTTAGTTGGTCACTTCGCCCACAGTGAACCTATGTGTCAAGTCCTG
GCTGGGTGCTGGACCTCTGGAGCTGCACAAGACCCAGGCTCTGGCCCCCAGGACAGCTGGCTGGGAGGAA
GAAGCAGCCCTCAGGCTTGAGAGATACCCAAATGCTGAGATACTGAAGGCCAAGAGCATCAGGGCCTGAG
CAGCAGGTGCTCCATGCCTTTGCAGTAGCAGTGCCCTCTGCCAGGGACTCCCTGGTCCTGGGCCGCCTGG
TTGAGATGTCCAGGCTGGACTGATTGGTTCCTGTACGGGCACTGGTTGAGCTGTTCTCCTCTTTGCACAG
ACAGCCTCCTTGGAGGAGCTGTAACTGGCTTGTCCCCCAGCCTGGGACAGGGCTGGGGCAGTGGAGCTAG
GGTGGTTTCAGTGTAACTTTCCACCTAGGGGTGGCCCCACACCAGTGGCTCTCACTGGCAAACTCTCCGG
GCTCCTGAGTCTCCCTCTTTTATTTTATTTTTTCGAGACAGGGTCTTGCTCTGTTGCCCAAGCTGGAGTG
TAGTGGCGTGATCATGGCTCACTGCAGCCTCAACCTCCCAGGCTCAGGTGATCCCCTCAGCTGGGACTAC
AGGCATGCCACTATGCCCTGTTAATTTTTTTTTAAATTTCTTGTAGAGATGGAATTTCATTATGTTGCCC
AGGCTGGCCTGGAGCTCCTGAGCTCAAGCGATCCATCCGCCTTGGTCTCCCAAAGTGCTGGGATTACAGG
TGTGAGCCACCGCATCCAGCCTATTTTTTATTTATTTTTGTTTTTTATTTATTTATTTTTTTGAGATGGA
GTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGACTTGATCTCAGCTCACTGTAACGTCCACCTCCTGGG
TTCCAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGAGTGTGCCACCACGCCCAGCTA
ATTTTTGTATTTTTAGTAGAGACAGCGTTTCACCATGTTGGTCAGCCTGGTCTCGAACTCCTGACTTCAA
GTGATCCTGTCTTGGCCTCCTAAAGTTCTGGGATTACAGGCATGAGCCACCGCGCCTGGCCTATTTATTA
TTTTTATGTCTCACTCTGTCACCCAGACTGCAGTGCAGTGGTGCGAACACAGCTCACTGCAGCCTCGACC
TCCTGGGCTCAAATGATCCTCCCACCTCAGCCTCCCAAGTAGCTGGAACTACAGATGTATGCCACCGTGT
CCGGCTAAGTTTTGTATTTTTTGTAGAGATGGGGTTTCACATGTTGCTCAGGCTCACCCCATCTTTTATT
TTTAGAGACAGTCTCACTCTTGTCACCCAGGCTGGAGTGCAGTAGCACGATCTTGGCTCACTGCAACCTC
TGACTCCCGGGTTCAAGCAATTCTCCTGCTTCAGCCTCCTGAGTAGCTGGGATCGCAGGCGTGAGCCACT
GCACCCGGCCTTAGTCCCCCTCTTTTAGCTCTGGGGTAGTGGGAGCCTCCAACAGCCCTGCAGCACCCAG
CAAGTCAGCTGTGGCCTCCCTGGGCCTCTGGTCCTGGCTCTGCCTTGCCTCATGCTGGCTCCACACAGCC
CCCCAAATCTACCTCTTCTTTGTGGGCAGCCAGGCCTGCCCTCGGGTGGCACTCTGGGGCTAGCCAGTGT
GCCTACAATTTTTTCCGTATTTGGCCCCAGAGTGGGCTCCTGGGGAGGGGGCAGCATGCCAGCCTTGCTC
CCTGCTCTTCCATTTGGTAAGGCTTAGAGGCAGGAGGCCTGGCCCCAAGTTGTCCTGTGGAGGGTCCTCA
CAGTGGGTCTTGGCAGCTGCCCCTCCAGGCTCACCTTCCTGCCCTTGCCACCTCCCACAGGGCCCCTGCG
CTTTGTCATCATCCACAAACGCTGCGTCTACTACTTCAAGAGTAGCACCTCTGCCTCCCCGCAGGGCGCC
TTCTCCCTGAGTGGCTATAACCGGTAAGTGCCCGACCTGCCTGCTGACCTCGGGCCCCCACAGCCAGCGG
GTCCCTCCCAGCAGAGCCCCAGCTGGGCCTGCCCCTTATTCCCGCCCAAGGAAAGTGCTTTCCCGAAGCA
GCCTTCGTGCCCATCCCCTGTCCTCCCCGCCCAGCCTCCACAGTGCCTCGCCCTCTCCGCGTGTCCTGCC
CTCAGGCGCAGGACCTTGGGGGTGCCAGGGTCTTCCTGTGGCTTTGTGCACAAGTCCCAGAGGCCTGGAC
AGCCTGCTCTCCAGAGTCTCCACTCGGCCACCACCCAGTGACTTCTGCATCTCTCCTCCCTCCCCACCTT
GCCCCTTCACCCACCATGACCTCTTTCCAGGCAGCCCAGACCCCAGCCAGCCCAGGCCCTGTCCCAAACA
GAGGCTGGGCCCTAGGATTCCCTGCTCCAGGGTCCTCCCAGGCCGGGATCAGTTTCCAGCTTCACGCATG
TCCTGGGCTGGGCTCTATCCAGTGATGGGCCATGAGCTGCCCTGGAGGGCCTTCTGCACTAACAGGCAGG
TGGGCGGTGGCCAGGTGGCATGTGGGTGGGGGAGTGCACTGGCACAGCCCATGGCCTGGGGCCCACACAA
AGGGTTAATGCTGGCACCCAGCTCTGGTTCTGGTTGCTATCCCATCGGAGCCCTGCACCCCACAGCATTT
ATCTATGCCTCTTACCACCCCTTAAGGGCCCAGCCCCTCCTGCCTATGAGGAACCAAGGGTCACATGGTA
TCAGTAGTGGAACTGGGACCTTTGGAGTGACTCTAGGCCCCGGCCTGCACCCTGGCCACAGGCCTGGTAG
GGCAGCCTTGCCAGAGCACCCAGCCTGGTGCCAGGCCCCCATGCTGGCACAGAGCTCCGGGGTCCCCTGG
GGAAGGTCGTTCCCTTTCTGCAGACAGACACAGCGCCTTCCGGCCTCCAGCATGTGCCAGCCATGCCATG
AACCCTGGGCCCAGTGGCAGATGCAATCATGCCCCAGGGAATTGTCTAGCCCATCAGGGAATGCTGGGCC
TGAGGAGTTTTGGCCAGGCTCTTATGGGCCGTATAAGTGCACCTGCCCCGGTGCCTGGCTGGCAGGGCTC
CCCACGTGATAAACTCAGCGCAAACCCCACATCCTGGCTGTGTGAGTGGGCCGCAGAGCAGGCACGAGTC
ATTCCCTGAAATGCACTCAGGGCTGCTGCTGGGCCTCAGGGGCACGGAGCCCTGTGGGGTACCCCAGGGA
GGCCAAGCAGGCCTGGGCAAATGGGGCCAGAGGTGGGAGGGTGGCGTCCATGTAAAGTGGCATTGGCGGG
CATCGAGGCAGGTTTGTCCTTGTCTCCTGTGCACTGCTGCTCCCACGAAGCCCACCCAGCACCCCTCTTC
CATGCCCCTCCATGCCCCCTTACGCCTGCCTGGGCATGAAGCTCACTACTGGGAGCATCAGCAGCCAGGG
CCCACCCAGATCTGCCCTCTTCCGTTGGGGCGTGGTGCTGGTGCCGAGACGTGTTCACAGGGGGGTGCTG
TGGGAAGGCCATCCCTATAGCTGTGAACCAGGCCGTGACCCCTGGCGCTGTGCCCCCAGGGTGATGCGGG
CGGCTGAGGAGACCACGTCCAACAACGTTTTCCCCTTCAAGATCATCCACATCAGCAAGAAGCACCGCAC
GTGGTTCTTCTCGGCCTCCTCCGAGGAGGAGCGCAAGGTGACTGGGGGTCCGAGGACGAGTGCAAGGTGA
CTGGGGGTGTGGGCCTGCAGGCACCAGGCTGGACCTGCCTTGGGGGCTCGCTGGTCCTGGGGCGCTGGCC
TCTCAGCCCCGGGCAAAGAGAAGGTGTGGCTGGGACACAGGCAGCTGGGTGGGTGCTGCCCCAGCTCCAT
CCCCATGCCCAGAGCCTGGTGCCAGCGCCCACACAAGGAACATGCTGTCCTGGGAGGTGCCCCTGTGGGC
TGAGGGGAGCCACACAGCCATGTTGTATCCAGCCGGGTCGCCTCCTCTGACCTTGGGAGTCACAGCAGAG
CAGGCAGGGCAGTGAAGGTGGAGGGGTGCGGTGGGGCCCACCCTGGTGGCACCGTGCCCACCACAGCCCC
GCTGACCTGCAGAGCTGGATGGCCTTGCTGCGCAGGGAGATTGGCCACTTCCACGAAAAGAAAGACCTGC
CCTTGGACACCAGGTGAGCCCGGGCCCAGGGCATACCGGGCAGTGAGGGTCCCTGGGGCGCCTGGGCCTG
ACCCGGGTGTCCGCCTCCCAGCCCCGGGCTTGGCATAGAATTCCGTCCTTAAAGGTTTCCTGGAGGTGCC
AGCTGGGCTGCGTCTCTCTGCTCCTGTCTACAGATAAACACAGATACAGGACAAGCACAGATGTTCACAC
ACAGACGAGCGACACGCGGACACGCACACACAAACACAAGCAACACGCGGACATGCACACACACAGAGCA
ACACGTGGACATGCACACACACACAGAGCAACACGTGGACATGCACACACACACAGCAACACGTGGACAT
GCACACACACACAGCAACACGCAGACATGCACACACAAACACAGAGCAACACACAGACATGCACACACAC
ACAGAGCATGCACAGGTGTGCATGCATCTCTCTGCTCCTGTCTACCTCCAGCCCCATCTGTCACATCTGT
TTCTGTGTTGCCGCCATGGGTAGTTCCTGTTAGGGGATCATGTGTGTTTATACGCCTGCCACCAGGGAGG
CCTGGCATGGCAGCAGCTGGGCACTTTGGGTTCCCAGCCCCCTACGCCCACCCTGGCTCACTCAGAGTGA
GACCAGAATGATTCTCCTGGCGGGGCGCCACTGGGCAGTCTGAGCCTTGGCAGCCTCCCCCGTGAGCTGG
CCCGACGTGCAGCAGATACTCTCTGGATTGGGACTCCCTGGCCCGAGACGATGTGGAGGGAACAAACCTT
CCTGTCCTTTCACAGGTGGGGACAGGAGGCCCAGGGAGGGCTGGGCACAGCCAAGGCTGCCCTGCAGGTC
CCTGCCTGACTCCGGGCTCCCGTCCCCTGGTCCAAGCTGTTCAGCCTCGGGCCCAGCCCAGAGTCGCCCA
AGCCCTCTTGAGGCAGGAGCAGGTGGAGACGTGGGCACAGTCAGGGCGGCCATCCTTGTGGGCCAGAAGG
GGCACCTAGTGGAGGCACCACAGCCTCCAGTTCTTCTGTCTGTCCTTGTAACTGTGTGTGCATGTCCGCG
TGTTGCTCTGTGTGTGTGCATGTCCGCGTGTTGCTCTGTGTGTGTGTGTGTGCATGTCCACGTGTTGCTC
TGTGTTTGTGTGTGCATGTCTGCGTGTTGCTCTGTGTTTGTGTGTGCATGTCCGCGTGTTGCTCTGTGTG
TGTGCATGTCCACGTGTTGCTCTGTGTTTGTGTGTGCATGTCCGCGTGTTGCTCTGTGTGTGTGCATGTC
CGCGTGTTGCTGTTTGTGTGTGCATGTCTGCGTGTTGCTCTGTGTGTGTGTGCATGTCCGCGTGTTGCTC
TGTGTGTGTGTGTGTGCATGTCTGCATGTTGCTCTGTGTGTGTGTGCATGTCTGTGTGTTGCTCTGTGTT
TGTGTGTGCATGTCTGCGTGTTGCTCTGTGTGTGTGTGCATGTCCACGTGTTGCTCTGTGTGTGTGCATG
TCCTCATGTTGCTCTGTGTGTGTGTGCATGTCCGCATGTTGCTCTGTGTGTGTGCATGTCCGCGTGTTGC
TTGTGTTTGTGTGTGCGTGTCCGTGTGTCGCTCGTCTGTGTGTGAACATGTGTGCTTGTCCTGTATCTGT
GTTTATCTGTATACTTCCATGTCTGTGTGACAGAGTCCTTGTGTCTGTGTGTCTACATGTCTGCGCGTGT
CCCTGTGTCTTTGTGTATATATATCCATGCCTGTGTGCCTGTGTTCCTGCGTGTGCTTGTGTGTGCACGT
GTGCATTTGTGTGTTTGTCAGAGTATGTGTGCATGTGTGTGTCTGTCAGCGTATCCATGTGTGCATGTGT
GTGTCTGTCAGCGTATCCGTGTGTGCATGTGTGTGTCTGTCAGCTTAACCATGTGTGCATGTGTTTGTCA
GTGTATCCGTGTGTGCATCTGTGTATCTGTCCATGTATCCGCGTGTGCCTGTGTGTACCTTTGTGTGAGC
ATCAAGGGACCTCCCAGGCCTGGTGCTCACCGTCCGCCCCAACGCACCCTGCATTGCAGCGACTCCAGCT
CGGACACAGACAGCTTCTACGGCGCAGTTGAGCGGCCTGTGGATATCAGCCTTTCCCCGTACCCCACGGA
CAATGAAGGTGAGGTCTTTCTCCGCATCCACTGCCCGTTCGCCTCTCCCCACCTGGCCTCCTCTTGGCCG
CTGTGGAGGAGAGGGAGGTGTGTTCGGCTGTGCTCCTTGCTCTGGCCCTTTGGCAGTGCGCAGTAGCATC
CCTGGGCTCTGGCCTTCAGCGGCAGGGTCTGGACTCACAGTCCTCCCAGCAGGTGCTTGCCCCTTGCCTC
CTGGACTCAGCCCCATGCATGGAGCATTGCTCGGAGACTGGGCCTGGGCCGGTTTGGCTCTCACCACCCC
CCTCTCCCCATGCAGACTATGAGCACGACGATGAGGATGACTCCTACCTGGAGCCTGACTCCCCGGAGCC
CGGAAGGCTTGAGGGTAGGTGGGGCGGGTGGGCCCGGGGAGCTCTGGGTGTGTAGGAAGCAGGGGCTGGG
ACCGGGAGCAGAGCCCCTCCCAGGCTGGGGATGCTGGCCCAGGTACCGCTCTGGGTGGCTTCCGCTTCCC
TGCTGTGTCCCAAGTATTATGAGGATTGGAGCAGAAGAGGTGTAGCTGCTGCCACCTCTGCTCATTTCCT
CCTCCCACCTCCCAGGAGGAGGGACAGGCACATGGCGATAAGTGGCAGGTGGAGCCGGGGTGGCCCTGGG
GGTTAAGCATGGGGAAGGAAGGACGCTGAGGAGCGCGCAGGCTCAGGGCAGGTCCATGTCCCCATGGCTG
CCAGCTTGTGTGGACACTGCTGCAGGCCAGGTCGTTGTCCCACCCCACCCCATCCCATCCCAGTGTCCAC
CTACCTCCTGCAGCACATGGCCTAGTCAAAGAGACAGGCTGATACAGGTGGTCACACGTGGATGACGGAA
GCACGAGCTGGGTGGGGAGGACGTGTCTCTGTCCTCCTCTGTCCCCTCCCTCCCTTCCTCCCTCCCTATT
GTCTCTCCAGCCCCCTCATCTTGTCAATTTCCTCATGTCTGGGGCTGATCCTTGATCCTTCCATTTTCCC
TGCGTCCGCCCTGCCTCCTTGTTTTCCAGCCTCCTGTTCTCACTGACTTGGCCTCTGCTGACCCCAGCCC
CTGACCCCGTATCCTCTCTGCCTCCCATCCCCTCCCACCTTCAGGCTCCCGGGTCCAGCCCCTCTTCTCC
TGCCGCTCCCTCCCTCCTTCCCTCTGGGGTCCCAGTGCCAACAGTCCTTCAGCCCACCAGGGTCTGCGGG
CCGCTTGCCTCAGTTTCCTTCCATGCAGAGCTCTTGTCTCACAGTGTTCACCATGCCTGTCCCTTCGCCC
ACACACTACTGCCTTCCGTCTTAGCCTGGCTGGCCAAGCCTTGGTGAACTCAGACTCCGTCTGCCCAGCC
CTGAGCTGCAGCCCAGTGAGGCTAGAGATGGGCCTGGGGAGCCACCAGTGCTCCTGCATGCTACTGCTCC
ACTCACCCTCCCTCTGTACCCCCTTCATCCTCTCCCCGAGACATTTCTCGTCCAGGACGCCTGTGGCCTC
CCCATTCCCAGTTCCAGCGCTCAGTCCTGAGCCACACCAGCCTGGCGCCTGCCCTGTTCCTTGCCAGCCC
GTCCTCCAGGCGCCCCGTGCTCCCAGGGTCCTCTTTCTCCTCAGCTCCTGTGCTGGCCCCATGAGGGATA
CCCGGGACTCAGACCTCACTCCTCTCCCGAATTCCCTGGGTGACAGCCTGGGTCATCTGCAGGCTGCCCG
CTCCGAGTCATGTCCAGCCTGGATTTTTGAACTCTGCACACATTCCCTGACCCCGCATCCCCTGGCATCT
CCCAGCTTTCTGGATATGTTCTGAGCAAACACGGGCCTTCACCTTCCTCCCAGCTGCTCCCCTCCCCTGT
CCTTGGGAATTCCGTCCTCCCTCTCGCTCCAGCCGAGCCCTGAGATCCTTCTTGACTTCTCTTCCTTTCT
CTTCCTTCCACCTCTGGGAACCTGATGGGCTCCTCAGGTGTCCTCTAGGCTTCAGGAAGCGTCCACAGGC
TGCCATAGGGAGGATGAATGGGGAAGGCTGGGGGTGGTGGGTGGTCTGGGACCCTGGGGAAGGCAGGATG
GGAGTGCTGGGTGCTGGGCTGCTGGGTGGGCAGGCTGTGGGGTGGGCCTACCATGGGTTGCACTCCTGGT
TGGCCTGGCTGACCACTGCCAGCAGAGGATAGTGTTGGCCCAGTCTCTGTCAGGGTCCAACCCGGGTCTC
TTTGCTCTGCAGATGCCCTGATGCACCCACCGGCTTACCCACCACCCCCAGTGCCCACGCCCAGGAAGCC
AGCCTTCTCTGACATGCCCCGGGCCCACTCCTTTACCTCCAAGGGCCCCGGTCCCCTACTGCCACCCCCG
CCCCCTAAGCACGGCCTCCCAGATGTTGGCCTGGCGGCTGAGGACTCCAAGAGGGACCCACTGTGCCCGA
GGCGGGCTGAGCCTTGCCCCAGGGTACCTGCTACCCCCCGAAGGATGAGCGATCCCCCTCTGAGCACCAT
GCCCACCGCACCCGGCCTCCGGAAACCCCCTTGCTTCCGGGAGAGTGCCAGCCCCAGCCCGGAGCCCTGG
ACCCCTGGCCACGGGGCCTGCTCCACTTCCAGTGCTGCCATCATGGCCACTGCCACCTCCAGAAACTGTG
ACAAACTCAAGTCCTTCCACCTGTCCCCCCGAGGACCACCCACATCTGAGCCCCCACCTGTGCCAGCCAA
CAAGCCCAAGTTCCTGAAGATAGCTGAAGAGGACCCCCCAAGGGAGGCAGCCATGCCCGGACTCTTTGTG
CCCCCCGTGGCTCCCCGGCCTCCTGCGCTGAAGCTGCCAGTGCCTGAGGCCATGGCGCGGCCCGCAGTCC
TGCCCAGGCCAGAGAAGCCGCAGCTCCCGCACCTCCAGTGAGTTTGTGTGGCGGCTGCAAGCCCTGCCTC
CAGCTACAGGGACCCTGGCCTGGCCTCTGACGGGCACTCTGCCCACCTCGCTCCCCTGGCACTCTTAGCC
CACGACGGGGTACCAGGTGCAAGGGTAGGAAGGTTCCCGGTTGCCTCCAGTCCGCCTTGGTGGAACGTGG
AGCATGTCCATGACTCAGCCCTGAAGCTTGCTGGAAATGCTTTGTTTTTTTTTCTTTTTGAGACAGGGTC
TCACGCTGGCAACCAAGCTGGAGTGCAGTGGTGCGATCTCAGCTCACTGCAACCTCCACCTCCGGGGTTC
AGGTGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCACCTGCCAACACGCCCGGCTAATT
TTTGTATTTTTAGTAGAGACAGGGGTTTCACTATGTTGGCCAGGTTGGTCTTGAACTCCTGGTCTCAAGT
GATCCGCCTGCCTCGGCCTCTCAAAGTGTAGAGATTACGGGCGTGAGCCACCGCGTCCTGCTGAAATGCT
TTCTGCTTTATTTCTGGGAAGTGCCAGGAGACCAGTTTGTCCCCCACTCCCACAGAGTTACGCCAATGGC
TGTGGTCAAAGGCATCTGGGACCCCAGGTGGGCTTCCTGCCTTGCCTGGCAGGCAAAGGGTCCAGGAACT
GGAGGGGCAGGTTTCGCATGGGTGTGCTGGCCGGCACGCGCACCCCCGAATGCCCTTGCCCACGGTGCCC
TTCTCCCAGGGCTCTGCGAACACCTCTGAGATGCCTGCTGCCTGTGTGGAAGTGCCTCTGAGCAGCCCTT
GTCACACTCGTGGTTCTAGTTCACCAAGGGCTGCAGAGATGCAGATGTTCGGAAGTCACGTTTTTCCATG
ACTGTGGGGATAGCGGTTCCAGGGCTCAGCCTCACCCACAGGCTGGCCTCCCATGCTGGCGTGGCCCGTT
AATCCTCCCTAGAGCCTTTGAGGCTTTGCTGAGCAGGACCGGCTAGCCACACAGGGAGGCGTGCAGGGAC
GCCATGGGCGTCCTTTGGGGTCACACTGCATTGTTCATCCAGTTCACGTCCACTCCCCTGTGGCTCTGAG
TGGGCTGTGATTTGGCCATGACCGGACCTCCCCTCCCCACGAGCATCCAGAGCACTTGTTGACGGTGTGA
TGACACTGAGCTTGGGAACCCTGGACTCCCTTGCCAGCATCCATGGCAGCCCTGACCCCTGACTCCGGTG
TCGGGCGATTCCTGCTGTCCCAGGGCAGTCAGCACGAGCCCTACCCGGATCTCTGTTGTCCTGAGCTTTT
TAGGGTCACAGGGGCCATAGCAGGCAGCTTGCCGTCCTCACACAGAGGGTGGAGTGGGGAGGGGAGCAGA
GGGTGGCCGCCCCGTGTCTGACAGTGAAATGGTCCTGCCTTCCTCTCCCTGCCCCTCCAGGCGATCACCC
CCCGATGGGCAGAGTTTCAGGAGCTTCTCCTTTGAAAAGCCCCGGCAACCCTCACAGGCTGACACTGGCG
GGGACGACTCGGACGAGGACTATGAGAAGGCAAGGCTGAGCGGCAAGCCTGGGTCCCAGTGGCCAGTAGG
TGGACAGGTGGTGGGAAAGCCATAGGCCAGGGCGGCCCCTCACAGACCGTCCTGAGCAAGGACCCCCCGA
GAACCCGGGAGCCTAGGGGGACACAGCACCATGTAAAGCCCCGGATCCCGGCACCGGGTGGCCACCGTCC
GGGGAGTGGTGGTGCGGGTGGATCACTCCGACGTTGGCACTGACACCGTCAGCCTCTTGCAGGTGCCACT
GCCCAACTCGGTCTTCGTCAACACCACGGAGTCCTGCGAAGTGGAAAGGTCAGCACAAAGCCCTGTGTGT
GCTGGGTCCTCCGCCATGCCCGGCTTCCTGCTTCTGTGTCCCTCTCACTAGCTTCCGTGTTGGGGAGTTG
CTGGCACAAGTTCATGGCCCTGCGTGCAGCAGAAACCAGAGGAGTGGACCTCCCTGCTCTGTCCCATGCC
CAGCTGGCACCCTGGCTGGCCAGGGCTCTGCTGGGCTGCTTCTGTCAGCCTCACGGCAGCCCGACGTGCT
CAGCTCCTGAGACCTACAACAGCGAGAGGACAGAAAGCCAGGCTTGGGAGCGGGGCGGGAAGGTCCGTGT
GAAAGCTGCCCGAGGAGGACTCACCCGCTAATATGACTGTCTTATTTTAGGTTGTTCAAGGCTACAAGCC
CCCGGGGAGAGCCCCAGGATGGACTCTACTGCATCCGGAACTCCTCTACCAAGTCGGGGAAGGTAGGCGC
CAGGGGAAGATGCCCCAGGGCCCCTCTGGCTCTCCACACACCCAGGCTGTGGGTGGGCCCAGGACTCCAC
AGTTCCTAAACCACTCCCCTTGTGGACTCTTACTTGGTGTCTCAGCTGAATTCTCTTCCCAGCAGCACCC
CCCAATCTATTCCCTAATAGATCTCCATCTGCAAATTGGGGGCCATGATGGCCACCTCCCAGTGGGGGCC
AGGACTCGGGTGGGGTAGGGCTCATGCTCTGGGGTGGCGGAGGCCACATGGGGCAGGGGAACGCCAGGTC
TGCCCGTGTCCCTCCGAGCGTGAAGTTGTGACCTCACTGAGCCATGCTCTCCCGGGTGTGTCGGAGTGGT
TATTGGTGGTTATGAGCTGTTGCTCATCAGAGGCTCTCTGAGGGCTGCCAGTGTCGTCCCCGCTGTCCTT
GTGCAGGTGGTCTGGAGTCCCTCCCCGCCCCCCGAGGGCCACAGGACCCAGCCTTGATGGTTCTGCCCCC
CTATCCCCAGCCCATGGTCTCCCGAGGCTGGTCCCGCTGTTTCTCAGGGAGCCGTCAGCCTCCCGCTTCC
GTCCTGTAGGTCCTGGTTGTGTGGGACGAAACCTCTAACAAAGTGAGGAACTATCGCATTTTTGAGAAGG
TGAGAGGGCTCTGAGTGGGACGGGGACCCTGGCCGCATGGCCTGGCAAGGGGCAGGGCAGAATCTCCCTG
ATGAGGCATAGGCAGCGGGTAGACTGAGACTGGCACCTCCAGGATACCGCCCTCCCCTTCCCCTCCACCA
TCGCTCACCCCCCACCCCTCCTGCTCAGCCTCCCTCCTCTCGTGGCCTACCTTTGTCCTCCACTGACCCT
AGTGGGGATGGGCGGTCAGCCATAGACCCTGGGTTGCTTGTCTTGTCTTTTTCTTTTTGCGGGGACAGGG
GTCTCACTGTCTTTCTCAGGCTGGTTTCAAATTCTGGGGCTCAAGCAATCCTCCCACCTCGGCCTCCCAC
AGTGCTGGGATTACAGGCGTGGGCCACCGTGCCTGGCCTAGGTTCATTTCCTGACCTTGTCTGAAGTGCT
CTGGGTGCAGGCTCCTGGACATGGAGGACGGAGGGGAAGTGAGGTGGGAACATGGAGAGCACAGGCCTGA
TGCGGAGGCCACCTTGGGGGCACCACCGACAGCCAGGGGCCAGCCTGGTGATGCCGCTGTTGATGCTGCT
GCCTTGTTTTACAGACGGGGAGACTGAGGCCTAGAGCCGCAGAGTGGCCTGGCCCTGCTGACGCTCCCCC
TTCTCTTCCCCCACAGGACTCTAAGTTCTACCTGGAGGGCGAGGTCCTGTTTGTGAGTGTGGGCAGCATG
GTGGAGCACTACCACACCCACGTGCTGCCCAGCCACCAGAGCCTGCTGCTGCGGCACCCCTACGGCTACA
CTGGGCCTAGGTGATGGCAGTCCATGTGGCTGCCAGGCCAAGGCAGTCACAGGGGCCCTGACCCCAGGCC
ACACAGACGGACATGGGCCCACATGGGAGGGTGAGCAGGAGCAAGGCTGTGCTTGCCTAGGGCCTCTGTG
ATGGACATCTCGTAGGACCCAGCCAGTCTCATCCAGCAGGTTGGGTTCTAGGGCTGAACCAGGCGCCAGG
CTCCAGAGGACGAAGGGACTCTGTTGCCCCACACTAACTTGCCCTGTCCCAATCCCAGAAACCCAGGACC
AAGCTGTGCCTGGGCTCCAAGGACAGGAACACTGGTCCCCCCATCACACTCACCCCTAAGTGGGCTGGGA
GCCAGGCAGGGCCAGGGCAGCTGGGTGGGGGCCGGGGCTGGCCCTGGGACCCCCAGGAACGCTAAGACAC
AGGCTCCAGTAGGGGCTGTTGCCTCCAATAAAGCAGCAGTGAGCTTTGCCTTGGTGGCTGGGGCTTGATT
GGGAAGGAGGGGATTACCAGCTTACTGGGTGCCCATGCTGATGTCTAAGTGGTGACCGCAGCAGTACCCG
GGAACCCCAACAGTTGGTTGTCTTGTCTTCCAGGGTGCAGGTCACTGAGTGACTTCCCCAGGGTGCACAG
CGAGTAACAGATCAGGACCCAAACTTGGGCAGTCTGGGCTGGGAGCCCACACCCCACTCACCAGTTCTGC
TGCCTCAGGTCAGGCCAGGGCAGTGCTGCTGCAGAGCTAGAAGGCCCTGCAGCTACAGCTGCTTCATTCC
CTGCATTAGTGCCTGGTTACTGGGTACCTCCTGAGTGGCTGTCCCCGTTCCAGAACTTGCATACACTGAG
CGGGCTACAGAGCTAGAAGGCCCTGCAGCTACAGCTGCTTCATTCCCTGCATTAGCGAGCAGTTATTGGG
TACCTCCTGCATGCCTGGTCCCATTCCAGACAGGGGCCTCTGGCCTGGCTGAGTTCACAGCCCAGTCTGG
GGACAGCTGGGTATGAGGTGCTTACGGCACAGTGTCCAGGGCAGCTGGGTGTGCAGGGACTGGGGGCTCC
CGGAAGATTTTTTGGAGGAAGTAACAGCTACGATGGGATGGGAACAGTGGACCCTAAGCAGGCCAAGGGT
GCGTAGGGACGGTGGTACCCAGATGCCCAAGTCTTCCAGGCAATACCTGGCTCAGGCCCAGCCCCAATCC
ATCCCCTTACTTTCTGCCATGGAGTTCCAGCAGGTCACTCTCCCTGGCACACCTTCCAGGCTGGATTTTT
AATGAAACAGACTCAGGGAGGTAGGGGCTGGCAGGGACCCTAGAATCCTTGTGATTTTTCTTAGCACCTT
ATGTCAGGGAAACCTAAACTGAGGTCAGCACTTGGGCCCACTGACAGTGACTGACTGGGGGAGAAGGTCC
TGCAGCCCCCTTCCCCTGGGTGTGTTCTGGGGACCTGTGGTTTGCTGGCGGAAACAAGTGATGAGGCTGG
TTAGCGGATGTGGGAGGCTGTGACCCCAGGGGGCCATAGGGTGCGGTGGAACTGCAGGCCCTGCAGATGA
CGGCAGCCAGCTGCTTCCAGGAACCAGGTGTCCAAGGCCACCTCTGCAGGGGTTTCCTCTTCAGCCTGCC
TGGGGTGAGAGGTCAGTGCACCACAGCCGAGGCTGGAGCACAGGGAGCTTCTGTTGTTCTGATCTATCTC
TGGAAAACCAGCCATTCCTCCTCCCTGCAGTCAGAATTCTTTGCCCTGTCTGACCTGAACTTGCTTAGGG
AGTCATGCCACTCCCCACTGTGGCCATAGTTTCTCTTCCTGTAAAATTTTATTATTTTAGTTTTTTGTTT
TTGAGATGTAGTCTCACCCTGTCGCCCAGGCTGGAGTGCAATGCCGTGATCTCCGCTCACTGCCACCTCC
GCCTCTCTAGTTCAAGCGATTTTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTCCAGGCGCCCGCCACCA
CGCCTGGCTAATTTTTTGTATTTTTAGTAGAGACGGGATTTTATCATGTTGGCCAGGCTGGTCTCGAACT
CCTGACCTCAGGTGATCTGCCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTGCC
TGGCCCCTTCCTGTAAAATTTTTAAATGGAGAATTGGGTGCGAGATGTGGTTTCCAGCCTGGTGCCTGGG
GTGCTGAGCTAGTGAGTGGTGCAGTCCAGGACACCTTTGCTTTATGTCACTTACACGGTCACCTGGAGCC
GGCTCAAGTGGCTAAAGCATCCTGGGGCCCAGAGCCAGGTGATAGGTCCCTCTGGCCAACTGGACAGTTG
AGGCCTGTGGTTACCCGAAGCCCAGCTGGGGCCCTGGTCCAGCCTCGCCTCCCAGACTCTGCACCTGCTA
GCACAGCTGTCCACGTCTGTGTGAGCTGCTCTAGGCCGAGGGCCTCAGTTTCAAGAGTGTGTTGGGGTGG
GATGGGGCAGGCCGTGGTCCTCCAGCATGAAGAAGGAGCCATGAGGAGTTCCCATGACCTCCCGAGACTT
GCCATAAGTGTTCTAGTCCACATATAAGGGTAGGGTTGGGATTACCATTTACTGACCACATCTGTGAGGT
GCCGAGCTGGGTGCTTGACATCATTTGCTTGGAGAAGCAGCTGCTAGTAGACCCATTTTACAGGTGAGAG
AACCAAGTCTCACAGAGGCCTGGGTTCAAGTCCCACCTCTGCCACTAACTGGCATGTGACCCTATCTATC
CTTCACTGCTCTGAGCCTAGACCCTGGCCCCTGCCTGGCTCCCTGCCAGGCTCCCTGCCACCCCTCACGA
CCTCTGATGGTCGTTGTGGGGGTCTCTTGCCTGGCTCCCAGGGCTAGGGTTAGGGCTCTGGAGGTGCTTT
CACTCAACCAAGGGGGCCACAGCACTGGGGAGTGAAACTGCCCCGCCTCACCCTGCGTTGCCCTCTGGGT
CTGTGAGGGTGGGCTGGCAGGAGGCCTAGGCCTTGCCCTAGGGGCAGTCCTGCTTCCTCATTTTATAGAT
AGGGAAACTGAGGCTTTGGGAGGACTCACTGACATACCTACCTTCAAGATGAGTTCAGGTGGGCTCAGTT
CTGGGGCTTGGGAAAAGGGCCCCAGTGGCTTTGGGAAGCACCCCCAGCCCAGGGTGAAACATGCTTCTTC
TCTTCCTGTGGTTCCATCCGAAGGATTGTGGTGAGCCCCGTGCCTTCAGTTAATAAAGATTTGTATTGTG
AAAAGATTTTTTCTTTTTTTTTTGGGACACAGTCTCACTCTGTCGCCCAGGCTAGAGTGGATTGGCGTGA
TCTCGGCTCAATGCAAATCTCCAGGGTTCAATCGATTCTCCTGCCTCACCCTCCCATGTAGCTGGGATTA
CAGCTGCCTGCCAAATTTTTGTATTTTTAGTGGAACCGGGGTTTCACCATGTTGGCCAGGCTGGTCTTGA
ACTCCTGACCTCAACTGATCCGCCCACCTTGGCCTCCCAAGTGCTGGGATTACAGGCGCGAGCCACGGCG
CCCAGCCTTGAAAAGATGTTTTTAGAACCAGAAGAAACCTCGGTTCCCACTGATCCTTCTGGGCCACGTT
GTGCGGAGCTCCCCTGCTGGTTGGGGCTCAGCGCAGCCCCAGGGAGGTGCTTCCTGCACCTCAGGATGGG
CGAGGGTGGGCATTGGGGGAGAGGGGGACCTGGGACCTGCGGCTTAGTTCCCTGAGGCAGGCAGGGCTTA
TTGGGGCCATTTCATAGAAAGGCAGATTGAAGCTCAGCAGGGAAGAGGCTTTTGAGGGTGATCCAGGCGC
TGGAGGGATGGCCTAGGACACCAGGGTCACACCAGGAACATGGGAGGGCCGTGCTTGTCTCTAGACGAGG
GGAATGGGGGAAGGGCCACAACCTCTGTTTCTGTGACCCAGCAGCATCAAGCCCCTCGCTGGGCACCTCG
CACACACCCCCTGCCTTATCTCTGCCTGCACGCCCTGTTCCCTCCACCTAGACTGCCTGCTGAGGGGGCA
GTGCCAGGAGGTTGCCTGTCCTTGGGGAAGAGGGGCAGTGACCCTGTGAAGATGCTTGACAGACAACCCC
CACCACCTCAGAAGTGTGTGTGAGTGGTGAACCCTTTTAAGCCATCTTCCAACCATTCTCACTGGAGGGA
GATTTGATGGGTACAGAGCAGACCCCTACCTGTCTACCCTCCTTCGGACCCCTAGGAAGCTTCGCAGGCC
TTCCAGGCTGCCAGACAGCTGCCCTGGCGTTGCCGTCTGCTTCTTCCCTGGCCCCACTCTGAGGGGCTCA
GAGCTGAGGCAGAATCCCTTTTTCATTCATTTCCTGCAGAATAAAACAACATACAGAAAAGTGAATAAAA
CATAAATGCACAACCTAACACACTGTTAGGAAGTGAACGATCTGCAACCACCATCAGGAAATAGTTTTGC
CAGCACCCAAGTGCCCTCCCCTCACAGTGTCACTTCCGGCCTCTCTGCCCTGGCTTATGTGAGTCTTGTG
TTCTTGTTTTTCTAAAAAGTCTTCAGCACCCAATTATGCAGGCATTGCAGTATTTTCCTGTTTCTGTGCT
TTATCCCCTTGAATCATACAGATGCAAATTCTGGCAGCTGGCTTCTTTGGCTCGTTATTATGTCTGTGAG
ATTTATTCATGTTGCTGTGCGTAGTATAGTTTGTGCATGTTCATTGCTAAAAACTTCCATTGTTTGGCTG
TATCGTAGTTCACAGATTCATTTCACTGTCAGTCAAGCTTGTCCAATGCATGCAGCCCAGGATGCCTTTG
AATGTGGCCCAACACAAATTTGTAAACTTTCTTAAAACATTATAAAGATTTTTGTTTGCGATTTTTTTTT
TTAGCTCATCAGCTATAGTTAGTGGTAGTGTATTTTATGCGTGACCCGAGACAGTTCTTCCGGTATGGTC
CATGGAAGCCAAAAGATTGGACATGCCTGCTGTAGATGGACAGTTGGTTTGTTTCTAGTTTGGGGTAACT
ACACACAATGCTGCTAGCAACAGTTTTGTCCATGTCTCTGATGCACGTGTGTTTTTTGCAAATGGTGCAC
AAATTTTTCTAGGGTTTGTACTCAGGAGTCTGACTCCTGGGTTCTAGGGTATGAAGATCTTTCTAAATAT
TGTTCTAGTTTACGTGCCCACCAGCAGTAAAACAGAATTCCCTTGCCTTCCCATCCTTGGCAGACATTTC
ACTTTTGCCAGTCTGGTGGGGTGTATAGTTATGGCCTTAATTTGCATTTAGCTAATTACCAAGGAGATTG
AGCATATTTTTATGTTTTTATTAACCATTTTGATTTTGTCTCCTGTGAAGTGTCTATCATCTTTTGCCCA
TTTTTTAACTTGTTGTCTTTTTCTTTTTCTTTTCTTTTTTTTTTTTT
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