Haynes Lab:Notebook/ASU iGEM/2012/06/20

6-20-12

• Plated negative control on LB Amp plate
• Liquid cultures of T7 promoter and constitutive promoter
• Transformation (LSE)

• Transformed DNA:

• RBS (well 1:1H BBa_B0030)
• TetR GFP (well 2:8A Part:BBa_I13522)

• Cells: DH5 alpha (donated)
• Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation)
• Controls: puc19, no DNA