Haynes Lab:Notebook/CRISPR Editing/2014/09/02

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09/02/2014

Miniprepped colonies picked yesterday from ligation plates that were left out for 72 hours.

Sample Read# 260 280 260/280 ng/┬ÁL
gRNA 260.0280.0132.10227.876
gRNA 300.0220.0082.55621.644



Ran 3a and 3b PCR products on gel, both bands were there. 3a was really bright but both lanes had lots of background.
Purified the PCR products using Sigma kit.
PCR

Sample Template F primer R primer Exp band
4a3aP179P182962
4b3bP179P182962
4c3bP192P195918

Annealing Temp 55, et 1min


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