Haynes Lab:Notebook/CRISPR Editing/2014/09/30

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09/30/2014

Annealed 4 reactions each of

' ul for 400ng ul elution solution ul water ul 10x annealing buffer
gRNA 20 Luc2.860.1461
gRNA 20 +dox 11.961.0461
gRNA 20 +dox 22.230.7761
gRNA 29 Luc2.520.4861
gRNA 29 +dox 12.610.3961
gRNA 29 +dox 22.040.9661
Luc143.05-0.0561


Added two of each with 0.5ul Surveyor and 1ul enhancer, added 1.5ul of water to the other two of each, incubate at 42deg C for 45min.
Ran one of each on a gel



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