Haynes Lab:Notebook/CRISPR Editing/2014/10/22

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10/22/2014

Sequencing

Seq sample name Template Primer Primer name F/R Distance from gRNA
1 Luc14 gRNA 20 Srvyr PCRP197SRVYR Luc F2F390
2 Luc14 gRNA 20 Srvyr PCRP160gRNA 029 ES P RR238
3 Gal4-EED +dox 1 gRNA 20 Srvyr PCRP197SRVYR Luc F2F390
4 Gal4-EED +dox 2 gRNA 20 Srvyr PCRP160gRNA 029 ES P RR238
5 Gal4-EED +dox 1 gRNA 20 Srvyr PCRP197SRVYR Luc F2F390
6 Gal4-EED +dox 2 gRNA 20 Srvyr PCRP160gRNA 029 ES P RR238
7 Luc14 gRNA 21 Srvyr PCRP197SRVYR Luc F2F259
8 Luc14 gRNA 22 Srvyr PCRP197SRVYR Luc F2F341
9 Luc14 gRNA 22 Srvyr PCRP160gRNA 029 ES P RR284
10 Luc14 gRNA 27 Srvyr PCRP169gRNA 026 US P FF172
11 Luc14 gRNA 27 Srvyr PCRP198SRVYR Luc RR247
12 Luc14 gRNA 29 Srvyr PCRP149gRNA 020 ES P FF238
13 Luc14 gRNA 29 Srvyr PCRP176gRNA 030 US P RR195
14 Gal4-EED +dox 1 gRNA 29 Srvyr PCRP149gRNA 020 ES P FF238
15 Gal4-EED +dox 2 gRNA 29 Srvyr PCRP176gRNA 030 US P RR195
16 Gal4-EED +dox 1 gRNA 29 Srvyr PCRP149gRNA 020 ES P FF238
17 Gal4-EED +dox 2 gRNA 29 Srvyr PCRP176gRNA 030 US P RR195
18 Luc14 Srvyr PCRP197SRVYR Luc F2F
19 Luc14 Srvyr PCRP160gRNA 029 ES P RR
20 Luc14 Srvyr PCRP169gRNA 026 US P FF
21 Luc14 Srvyr PCRP198SRVYR Luc RR
22 Luc14 Srvyr PCRP149gRNA 020 ES P FF
23 Luc14 Srvyr PCRP176gRNA 030 US P RR



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