Haynes Lab:Notebook/CRISPR Editing/2014/12/17

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

12/17/2014

Gradient PCR of g029 treated Luc14 gDNA. 6 temperatures, 50ul reactions.

53°C
55°C
57°C
59°C
61°C
63°C

Ran gel, only saw additional bands at 53°C, didn't see any drop off in yield.
Set up 50ul PCR reactions for g023, g025, g030, untreated luc14 gDNA. annealing temp 63.


Personal tools