Haynes Lab:Notebook/CRISPR Editing/2014/12/18

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12/18/2014

Ran PCR from yesterday on gel.
PCR purified g029 PCR reactions. Yield about 30ng/ul. PCR purified PCR reactions from yesterday. Some were as low as 23ng/ul. Will redo

Sample Read# 260 280 260/280 ng/┬ÁL
g0290.0320.0162.00731.813
g0290.0290.0151.89929.381
g0230.0330.021.67433.467
g0250.0230.0121.93923.109
g0300.0230.0121.86222.585
untreated0.0230.0131.83923.346
all PCR for all gRNA treated cells, 2-50ul reactions gotaq at 61deg at.

Ran on gel
600
Extra bands on 31, 35, and 38. Will redo PCR at higher AT


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