Haynes Lab:Notebook/CRISPR Editing/2015/01/08

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01/08/2015

Ran the PCR reactions from yesterday on a gel. They look great, no extra bands.
Purified the PCR products using Sigma's PCR clean up kit. Eluted in 50ul elution solution.

Sample Read# 260 280 260/280 ng/┬ÁL
Untreated0.1070.0571.862106.778
230.0960.0511.88996.073
250.10.0541.846100.394
290.1040.0551.889104.281
300.0990.0531.86299.101
320.0930.051.86793.338
330.0910.0481.87590.77
340.0930.0511.84593.316
360.0940.0511.85493.841
390.0970.0531.84797.225
430.0890.0471.90188.782
440.030.0161.88930.391
450.0870.0471.86186.634
460.0790.0441.81479.106



Set up annealing reaction for the PCR samples

Sample ul for 400ng ul H2O ul anneal buff
Untreated3.759.751.5
234.169.341.5
253.989.521.5
293.849.661.5
304.049.461.5
324.299.211.5
334.419.091.5
344.299.211.5
364.269.241.5
394.119.391.5
434.518.991.5
4413.160.341.5
454.628.881.5
465.068.441.5

Put at -20 after they finished
Set up PCR wiht phusion for the remaining gRNA treated samples.


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