Haynes Lab:Notebook/CRISPR Editing/2015/01/23

Project name

Main project page Previous entry      Next entry

01/23/2015 Sent out untreated Luc14 gDNA PCR product of P197/P198 for sequencing: Label Primer Direction RD-1-3 P175 F RD-4-6 P161 F RD-7-9 P173 F RD-10-12 P159 F RD-13-15 P170 R RD-16-18 P156 R RD-19-21 P172 R RD-22-24 P158 R Quantification of editing efficiency with restriction enzyme' cutting 1μg of untreated and g033 and g034 treated Luc14 cells gDNA PCRd with P197/P198 and column purified samples from 14.12.06 experiment with PfoI at 37°C for 3 hours. Sample Read# ng/µL ul for 1ug ul H2O ul buffer ul PfoI Untreated 85.402 11.71 5.29 2 1 g033 78.942 12.67 4.33 2 1 g034 80.841 12.37 4.63 2 1 Quantification with 1% agarose gel