Haynes Lab:Notebook/CRISPR Editing/2015/01/24

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01/24/2015

Cell line gRNA Rep 260 280 260/280 ng/┬ÁL
Luc14g04510.1820.092.013181.634
Luc14g04520.2460.1222.012246.109
Luc14g04530.1750.0891.953174.603
GAL4-EED +doxg04510.0740.0362.05874.461
GAL4-EED +doxg04520.0830.042.07182.614
GAL4-EED +doxg04530.0590.031.96958.964
Luc14g04610.2550.1252.036255.228
Luc14g04620.1710.0852.01170.592
Luc14g04630.1440.0722.003144.251
GAL4-EED +doxg04610.0730.0381.92772.999
GAL4-EED +doxg04620.0790.041.95478.598
GAL4-EED +doxg04630.0770.041.92677.323



Plated for transfections
Used 12-well plates, have enough wells to do 3 gRNAs. Plated GAL4-EED+dox cells at 80% confluency.


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