Haynes Lab:Notebook/CRISPR Editing/2015/03/12

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03/12/2015

Sample Read# mL culture elution 260 280 260/280 ng/┬ÁL
KAH228210.120.0641.885120.452
KAH228220.0190.0092.05618.943
KAH228610.3830.2051.87382.939
KAH228620.0380.021.89438.18
KAH2281210.3760.2011.873376.089
KAH2281220.0440.0251.76243.806



Cut 250ng of g031 treated luc14 and gal4eed+dox P151/158 nested PCR products. one hour at 30degC then heat inactivated at 65 for 20 minutes. No enzyme control has 13ul water, 0ul enzyme:

water12.5
DNA5
Buffer 2
Bsa1I0.5
total20

Ran on BA, saw lots of uncut amplicons, equal for Luc14 and Gal4, think the enzyme didn't cut completely.


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