Haynes Lab:Notebook/CRISPR Editing/2015/05/19

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05/19/2015

Miniprepped colonies I picked yesterday from the ligation plate with KAH's cells. Sent them for sequencing with primers P220, 221, and 222 (1,2,3 respectively):

Sample Read# col # 260 280 260/280 ng/┬ÁL
pX330g30.1150.0611.897115.026
pX330g40.1440.0751.923144.187
pX330g50.0920.0481.92791.952



Tested the Neon. Tried GFP max at 0.5ug, 1.0ug, 1.5ug, and 2.0ug and the minipreps of pX330g.


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