Haynes Lab:Notebook/CRISPR Editing/2015/07/18

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07/18/2015 Finished gDNA extractions and first round pcr P197/198 on siRNA samples Ligated a bunch of grnas into pX330g following Zhang lab protocol. transformed 5ul ligation into 100ul Ryan's z-comp DH5alpha turbos, incubated on ice for 30 mins, heat shock at 42deg for 35s, ice for 2min, plated all cells. gRNA gn001 T gn001 B gn003 T gn003 B g005 g0013 g057 g058 g059 g060