Haynes Lab:Notebook/CRISPR Editing/2015/07/19

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

07/19/2015

Picked colonies for minipreps from new gRNA ligation plates

Picked colonies from previously built anti-luc grnas for minipreps

Froze down kah126 cells following http://openwetware.org/wiki/Haynes:CryopreservationMamCells, 12 vials worth. put at -80.

Added media to gal4-eeds in puro for freezing

Plated kah126 cells in 6-well plate for transactions with g003 tomorrow

Plated previously transfected kah156s for freezing and gRNA extraction

Pcr of mCh stop donors, 4-100ul rxns each

Nested pcr of re-opening experiment

g034 treated cells P149/1162
g048 treated cells P213/214


Personal tools