Haynes Lab:Notebook/CRISPR Editing/2015/08/27

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08/27/2015

Collected g034 transfections at 48 hours. Flow and gDNA extraction, three wells for g4+p and three wells for g4+d

Set up annealing reaction for Surveyor of Luc14 dilution of g034 treated cells collected 7/8/15.

Cell type drug ug g034 rep ul for 400ng ul buffer ul water for 15ul
Luc14-116.131.507.37
Luc14-126.031.507.47
Luc14-136.111.507.39
Luc14-0.115.981.507.52
Luc14-0.126.171.507.33
Luc14-0.136.601.506.90
Luc14-0.0115.871.507.63
Luc14-0.0126.081.507.42
Luc14-0.0136.871.506.63


Some PCR purifications from that same dilution experiment didn't have high concentrations so redid PCR with P163/215

Gal4-EED + dox 0.1ul g034 rep 3
Gal4-EED + dox 0.01ul g034 rep 1



Set up annealing reaction for Surveyor of Luc14 singlet from initial screen of gRNAs around the end of 2014

Luc14 gRNA 260 280 260/280 ng/µL ng/µL average ul for 400ng ul ann buff ul H2O
Luc14g0290.0730.0391.88976.35773.09674.735.351.508.15
Luc14g0300.0430.0231.88943.49243.38643.449.211.504.29
Luc14g0330.0840.0451.83780.73683.5682.154.871.508.63
Luc14g0360.0820.0441.8882.82582.08882.464.851.508.65
Luc14g0400.0650.0361.79463.73164.8764.306.221.507.28
Luc14g0410.0550.0291.88853.01154.68453.857.431.506.07
Luc14g0420.0450.0241.88547.20545.15746.188.661.504.84
Luc14g0430.0440.0231.8845.14343.89844.528.981.504.52



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