Haynes Lab:Notebook/CRISPR Editing/2015/09/10

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09/10/2015

Ran g031 P197/198 PCR samples on gel. No band in water-only control PCR. Gal4-EED +dox g031 rep 2 failed. Re-ran with 5ul of gDNA and it worked.
Nested PCR of g031 with P149/P174. No band in water-only control PCR.

gDNA extraction of g025 and g044 treated cells

Cells drug gRNA rep 260 280 260/280 ng/┬ÁL ul for 200 ul water 15-
Gal4-EEDpurog02510.0150.0081.97315.4512.942.06
Gal4-EEDpurog02520.0290.0151.99328.9696.908.10
Gal4-EEDpurog02530.020.012.03220.1019.955.05
Gal4-EEDdoxg02510.0370.0192.00637.2025.389.62
Gal4-EEDdoxg02520.080.0411.97480.3012.4912.51
Gal4-EEDdoxg02530.0750.0391.92674.5442.6812.32
Gal4-EEDpurog04410.0150.011.52214.52913.771.23
Gal4-EEDpurog04420.0230.0121.89323.3158.586.42
Gal4-EEDpurog04430.050.0252.00849.9794.0011.00
Gal4-EEDdoxg04410.0340.0162.10233.6195.959.05
Gal4-EEDdoxg04420.0690.0371.88169.0572.9012.10
Gal4-EEDdoxg04430.0440.0231.91744.0094.5410.46

Set up phusion PCR with P197/198 with 200ng gDNA


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