10/06/2015
Ran PCR of Gal4 siRNA g025 g034 samples with P197/198 on gel
- no bands in neg ctrl, all other lanes look great
Col pur Gal4 puro/dox g054 samples, set up annealing reaction
Cell type
|
drug
|
gRNA
|
rep
|
260
|
280
|
260/280
|
ng/µL
|
ul for 400
|
ul ann buff
|
ul water
|
Gal4-EED |
puro |
g054 |
1 |
0.1 |
0.054 |
1.87 |
100.189 |
3.99 |
1.50 |
9.51
|
Gal4-EED |
puro |
g054 |
2 |
0.09 |
0.047 |
1.91 |
90.466 |
4.42 |
1.50 |
9.08
|
Gal4-EED |
puro |
g054 |
3 |
0.105 |
0.057 |
1.862 |
105.5 |
3.79 |
1.50 |
9.71
|
Gal4-EED |
dox |
g054 |
1 |
0.104 |
0.054 |
1.919 |
103.727 |
3.86 |
1.50 |
9.64
|
Gal4-EED |
dox |
g054 |
2 |
0.115 |
0.063 |
1.825 |
114.985 |
3.48 |
1.50 |
10.02
|
Gal4-EED |
dox |
g054 |
3 |
0.092 |
0.049 |
1.891 |
92.038 |
4.35 |
1.50 |
9.15
|
Col pur Gal4 puro/dox g055 samples, set up annealing reaction
Cell type
|
drug
|
gRNA
|
rep
|
260
|
280
|
260/280
|
ng/µL
|
ul for 400
|
ul ann buff
|
ul water
|
Gal4-EED |
puro |
g055 |
1 |
0.068 |
0.035 |
1.93 |
68.229 |
5.86 |
1.50 |
7.64
|
Gal4-EED |
puro |
g055 |
2 |
0.072 |
0.038 |
1.87 |
71.618 |
5.59 |
1.50 |
7.91
|
Gal4-EED |
puro |
g055 |
3 |
0.079 |
0.042 |
1.882 |
78.826 |
5.07 |
1.50 |
8.43
|
Gal4-EED |
dox |
g055 |
1 |
0.07 |
0.038 |
1.839 |
69.741 |
5.74 |
1.50 |
7.76
|
Gal4-EED |
dox |
g055 |
2 |
0.074 |
0.039 |
1.875 |
73.714 |
5.43 |
1.50 |
8.07
|
Gal4-EED |
dox |
g055 |
3 |
0.074 |
0.042 |
1.773 |
74.442 |
5.37 |
1.50 |
8.13
|
|